Enzymes for the production of 2-keto-L-gulonic acid

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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435190, 435189, 435 691, 4351723, 4353201, 43525233, 536 232, 930240, 935 10, 935 14, C12N 121, C12N 904, C07H 2104

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059121610

ABSTRACT:
Mutants of 2,5-diketo-D-gluconic acid reductase A, an enzyme used to produce 2-keto-L-gulonic acid, a precursor of ascorbic acid (vitamin C) are prepared by site-directed mutagenesis. These mutants have increased catalytic activity, increased expression levels, and/or enhanced temperature stability.

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