Enzymes derived from thermophilic organisms that function as...

Details Enzymes derived from thermophilic organisms that function as... Enzymes derived from thermophilic organisms that function as...

2005-05-24

2005-05-24

Hutson, Richard (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S183000, C435S006120, C435S320100, C435S252300, C536S023100, C536S023200, C536S023700

Reexamination Certificate

active

06897053

ABSTRACT:
The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.

REFERENCES:
patent: 5192674 (1993-03-01), Oshima et al.
patent: 5242818 (1993-09-01), Oshima et al.
patent: 5322785 (1994-06-01), Comb et al.
patent: 5352778 (1994-10-01), Comb et al.
patent: 5374553 (1994-12-01), Gelfand et al.
patent: 5413926 (1995-05-01), Oshima et al.
patent: 5500363 (1996-03-01), Comb et al.
patent: 5545552 (1996-08-01), Mathur
patent: 5583026 (1996-12-01), O'Donnell
patent: 5618711 (1997-04-01), Gelfand et al.
patent: 5624833 (1997-04-01), Gelfand et al.
patent: 5633159 (1997-05-01), Pearson et al.
patent: 5736373 (1998-04-01), Hamilton
patent: 5744312 (1998-04-01), Mamone et al.
patent: 5789224 (1998-08-01), Gelfand et al.
patent: 5830714 (1998-11-01), Swaminathan et al.
patent: 5834285 (1998-11-01), Comb et al.
patent: 6066483 (2000-05-01), Riggs et al.
patent: 6100078 (2000-08-01), Riggs et al.
patent: 6238905 (2001-05-01), McHenry et al.
patent: 6395526 (2002-05-01), Uemori et al.
patent: WO 9109950 (1991-07-01), None
patent: WO 9315115 (1993-08-01), None
Ngo et al., Computational Complexity, Protein Structure Prediction, and the Levinthal Paradox, in The Protein Folding Problem and Tertiary Structure Prediction, 1994, Merz et al. (ed.), Birkhauser, Boston, MA, pp. 433 and 492-495.*
Rudinger, “Characteristics of the Amino Acids as Components of a Peptide Hormone Sequence,” In: Peptide Hormones, Ed. J.A. Parsons. University Park Press, Baltimore, MD, pp. 1-7 (1976).
Ngo et al., “Computational Complexity, Protein Structure Prediction, and the Ilevinthal Paradox,” In: The Protein Folding Problem and Tertiary Structure Prediction, Eds. Merz et al., Birkhauser et al., Boston, MA, pp. 491-495 (1994).
Thornton et al.,Current Opinion in Biotechnology6(4):367-369 (1995).
Wallace,FASEB Journal7:505-515 (1993).
McHenry et al.,J. Mol. Biol.272:178-189 (1997).
Yurieva et al.,J. Biol. Chem.272(43):27131-27139 (1997).
Bukhrashvili et al.,Biochimica et Biophysica Acta1008:102-107 (1989).
Elie et al.,Biochimica et Biophysica Acta951:261-267 (1988).
Pluthero,Nucleic Acids Research21(20):4850-4851 (1993).
Alonso et al.,Nucl. Acids Res.18:6771-6777 (1990).
Blinkowa et al.,Nucleic Acids Research18:1725-1729 (1990).
Carter et al.,J. Bacteriol175:3812-3822 (1993).
Chen et al.,Proc. Natl. Acad. Sci USA5211-5215 (1992).
Cullman et al.,Mol. Cell Biol.150:4661-4671 (1995).
Dong et al.,J. Biol. Chem.11758-11766 (1993).
Flower et al.,Proc. Natl. Acad. Sci. USA87:3713-3717 (1990).
Guibus et al.,Cell87:297-306 (1996).
Jack et al.,Cell55:447-458 (1988).
Kelman et al.,Curr Opin Gen & Dev4:185-195 (1994).
Kong et al.,Cell69:425-437 (1992).
Kornberg et al.,DNA Replication, 2nded. New York: W.H. Freeman com, pp. 165-194 (1992).
Krishna et al.,Cell79:1233-1243 (1994).
Kuriyan et al.,J. Mol. Biol.234:915-925 (1993).
Larsen et al.,J. Bact.176:6842-6851 (1994).
Lee et al.,Proc. Natl. Acad. Sci. USA84:2713-2717 (1987).
Maki et al.,J. Biol. Chem.263:6570-6578 (1988).
McHenry et al.,J. Biol. Chem.257:1657-1663 (1982).
O'Donnell et al.,Mol. Cell Biol.3:953-957 (1992).
O'Donnell et al.,Nucl. Acids Res.21:1-3 (1993).
Onrust et al.,J. Biol. Chem.270:13366-13377 (1995).
Onrust et al.,J. Biol. Chem.266:21681-21686 (1991).
Perrino et al.,Biochemistry29:5226-5231 (1990).
Studwell-Vaughan,J. Biol. Chem.266:19833-19841 (1991).
Stukenberg et al.,J. Biol. Chem.266:11328-11334 (1991).
Tsuchihashi et al.,Proc. Natl. Acad. Sci. USA87:2516-2520 (1990).
Tsuchihashi et al.,Gen. & Dev.6:511-519 (1992).
Tsuchihashi et al.,J. Biol. Chem.264:17790-17795 (1989).
Weiss et al., Cold Spring Harbor Symposia on Quantitative Biology, 52:687-693 (1987).
Yin et al.,Nucl. Acids Res.14:6541-6549 (1986).
Yuzhakov et al.,Cell86:877-886 (1996).

Affiliated with

Also associated with

Rating

Enzymes derived from thermophilic organisms that function as... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzymes derived from thermophilic organisms that function as..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymes derived from thermophilic organisms that function as... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3401958