Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...
Reexamination Certificate
2006-02-28
2006-02-28
Prats, Francisco C. (Department: 1651)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Stablizing an enzyme by forming a mixture, an adduct or a...
C435S280000, C424S094300
Reexamination Certificate
active
07005282
ABSTRACT:
Disclosed is an ionic liquid-coated enzyme, wherein the ionic liquid is an organic salt which presents as a liquid phase at a temperature of about 150° C. or below. The ionic liquid-coated enzyme according to the present invention remarkably improves enzyme functions, such as enantioselectivity and stability, when the enzyme which may be lipase is coated with an ionic liquid. Further, even in the case of when the ionic liquid-coated enzyme is reused, the enantiomeric excess, enantioselectivity, and activity are not degenerated. The coated enzyme is usable as a catalyst for providing a chiral intermediate required in the synthesis of chiral pesticides, medicines, natural chemicals, and so on.
REFERENCES:
Kwang-Wook Kim, Boyoung Song, Min-Young Choi, and Mahn-Joo Kim. 2001. Biocatalysis in Ionic Liquids: Markedly Enhanced Enantioselectivity of Lipase., Organic Letters, 3(10): 1507-1509.
Wasserscheid, Peter and Keim, Wilhelm. 2000. Ionic liquids—New “solutions” for transition metal catalysis., Angew. Chem. Int. Ed., 39: 3772-3789. Pertinent p.: 3776.
Mori, T. et al., “A Lipid-Coated Lipase as an Efficient Hydrolytic Catalyst in the Two-Phase Aqueous-Organic System”, Biotechnology and Bioengineering (2001), 76(2), pp. 157-163.
Okahata, Y. et al., “Enzyme-Lipid Complex. 9. Enhancing Enantioselectivity of a Lipid-Coated Lipase via Imprinting Methods for Esterification in Organic Solvents”, Tetrahedron: Asymmetry (1995), 6(6), pp. 1311-1322.
Markus Erbeldinger, Anita J. Mesiano, and Alan J. Russell, “Enzymatic Catalysis of Formation of Z-Aspartame in Ionic Liquid—An Alternative to Enzymatic Catalysis in Organic Solvents,” Biotechnol. Prog., vol. 16, Dec. 31, 2000, pp. 1129-1131; and.
Kwang-Wook Kim, Boyoung Song, Min-Young Choi, and Mahn-Joo Kim, “Biocatalysis in Ionic Liquids: Markedly Enhanced Enantioselectivity of Lipase,” Organic Letters, vol. 3(10), Mar. 17, 2001, pp. 1507-1509.
Kim Mahn-Joo
Lee Jae Kwan
Baker & Botts LLP
Fernandez Susan E.
POSCO and POSTECH Foundation
Prats Francisco C.
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