Enzymes and polynucleotides encoding the same

Details Enzymes and polynucleotides encoding the same Enzymes and polynucleotides encoding the same

2007-12-25

2007-12-25

Li, Q. Janice (Department: 1633)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S023200, C536S023600, C435S183000, C435S320100

Reexamination Certificate

active

10540759

ABSTRACT:
The invention relates to a multifunctional germacrene-D synthase and a polynucleotide encoding it. Included also are genetic constructs, transgenic organisms, plant selection methods and biofermentation methods. The enzyme may be used to prepare germacrene-D and/or other sesquiterpenes such as germacrene-D, delta-cadinene, gamma-cadinene, gamma-muurolene, gamma-elemene, delta-elemene, elemol or germacrene-B.

REFERENCES:
patent: 5264558 (1993-11-01), Kim et al.
patent: 5487983 (1996-01-01), Kim et al.
Guterman et al, The Plant Cell 14: 2325-2338, 2002.
Bohlmann et al, Proc. Natl. Acad. Sci. USA 95, 4126-4133, 1998; *of record in IDS.
Guo et al, Proc Natl Acad Sci 101(25):9205-9210, 2004.
Hult and Berglund, Curr Opin Biotechnol 14:395-400, 2003.
Aharoni et al “Terpenoid Metabolism in Wild-Type and TransgenicArabidopsis Plants,” The Plant Cell, vol. 15, 2866-2884 (2003).
Altschul et al, “Gapped BLAST and PSI-BLAST: A New Generation of Protein Database Search Programs,” Nucleic Acids Res. 25, 3389-3402 (1997).
Benedict et al, “The Cyclization of Farnesyl Diphosphate and Nerolidyl Diphosphate by a Purified Recombinant d-Cadinene Synthase,” Plant Phys 125, 1754-1765 (2001).
Bohlmann, et al, “Plant terpenoid synthases: Molecular biology and phylogenetic analysis,” Proc. Natl. Acad. Sci. U.S.A. 95, 4126-4133 (1998).
Bulow and Konig, “The role of germacrene D as a precursor in sesquiterpene biosynthesis: investigations of acid catalyzed, photochemically and thermally induced rearrangements,” Phytochem 55, 141-168 (2000).
Cai et al, “A cDNA Clone for β-Caryophyllene Synthase from Artemisia annual,” Phytochem 61, 523-529 (2002).
Cane et al., “Trichodiene Synthase: Mechanism-Based Inhibition of a Sesquiterpene Cyclase,” Bioorg. Med. Chem. Lett. 9, 1127-1132 (1999).
Chamblee et al “Identification of Sesquiterpenes in Citrus Essential Oils by Cryofusing GC/FT-IR,” J Essent Oil Res 9: 127-132 (1997).
Chang et al “Antimite Activity of Essential Oils and Their Constitutentsfrom Taiwania cryptomerioides,” J Med Entom. 38:455-458 (2001).
Chen et al, “Cloning, Expression, and Characterization of (l)-d-Cadinene Synthase: A Catalyst for Cotton Phytoalexin Biosynthesis,” Arch Biochem Biophys 324, 255-266 (1955).
Chen et al, “Cloning and Heterologous Expression of a Second (+)—Cadinene Synthase from Gossypium arboreum,” J. Nat. Prod. 59 (10), 944-951 (1996).
Benfey et al, “Regulated Genes in Transgenic Plants,” Science 244, 174-181 (1989).
Davis et al., “Cyclization Enzymes in the Biosynthesis of Monoterpenes, Sesquiterpenes, and Diterpenes,” Top. Curr. Chem 209, 53-95 (2000).
Davis and Essenberg, “(+)-6-Cadinene is a Product of Sesquiterpene Cyclase Activity in Cotton,” Phytochem 39, 553-567 (1995).
Davis et al, “Purification of (+)-fi-Cadinene Synthase, A Sesquiterpene Cyclase From Bacteria-Inoculated Cotton Foliar Tissue,” Phytochem 41, 1047-1055 (1996).
Guterman et al “Rose Scent: Genomics Approach to Discovering Novel Floral Fragrance-Related Genes” Plant Cell, vol. 14, 2325-2338 (Oct. 2002).
van der Hoeven et al “Genetic Control and Evolution of Sesquiterpene Biosynthesis in Lycopersicon esculentum and L.hirsutum,” Plant Cell 12 (11), 2283-2294 (2000).
Iijima et al “Characterization of Geraniol Synthase from the Peltate Glands of Sweet Basil,” Plant Physiology 134, 1-10 (2004).
Iijima et al “The Biochemical and Molecular Basis for the Divergent Patterns in the Biosynthesis of Terpenes and Phenylpropenes in the Peltate Glands of Three Cultivars of Basil,” Plant Physiology 136, 3724-3736 (2004).
Juteau et al “Antibacterial and antioxidant activities of Artemisia annua essential oil,” Fitoterapia 73, 532-535 (2002).
Kawasaki et al, “Specific Regulation of Gene Expression by Antisense Nucleic Acids: A Summary of Methodologies and Associated Problems,” Artific. Organs 20, 836-848 (1996).
Konig et al “The Sesquiterpene Constituents of the Liverwort Preissia Quadrata,” Phytochem 43, 629-633 (1996).
Lange, et al, “Isoprenoid biosynthesis: The evolution of two ancient and distinct pathways across genomes,” Proc. Natl. Acad. Sci. U.S.A. 97, 13172-13177 (2000).
Langenkamper et al, “Sucrose-Phosphate Synthase Steady-State mRNA Increases in Ripening Kiwifruit,” Plant Mol Biol. 36, 857-869 (1998).
Lesburg, et al, “Managing and Manipulating Carbocations in Biology: Terpenoid Cyclase Structure and Mechanism,” Curr Opin Struct Biol 8 695-703 (1998).
Llave et al, “Cleavage of Scarecrom-like mRNA Targets Directed by a Class of Arabidopsis miRNA,” Science 297, 2053-2056 (2002).
Luehrsen “Intron Enhancement of Gene Expression and the Splicing Efficiency of Introns in Maize Cells,” Mo. Gen. Genet 225, 81-93 (1991).
Maruyama et al., NCBI Entrez Nucleotide, Accession No. AF282875 (online) Jul. 25, 2001 (retrieved Apr. 6, 2004).
McIntyre “Strategies for the Suppression of Peroxidase Gene Expression in Tobacco. I. Designing Efficient Ribozymes,” Transgenic Res. 5, 257-262 (1996).
Meng et al “Coordinated Accumulation of (+)-ä-Cadinene Synthase mRNAs and Gossypol in Developing Seeds of Gossypium hirsutum and a New Member of the cad1 Family from G. arboreum,” J Nat. Prod. 62, 248-252 (1999).
Mozuraitis et al “Germacrene D Increases Attraction and Oviposition by the Tobacco Budwarm Moth Heliothis virescens,” Chem Senses 27, 505-509 (2002).
Napoli et al, “Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes In trans,” Plant Cell 2, 279-289 (1990).
Needleman et al. “A General Method Applicable to the Search for Similarities in the Amino Acid Sequence of Two Proteins,” J. Mol. Biol. 48; 443-453 (1970).
Niebel et al “Post-Transcriptional Cosuppression of β-1,3-Glucanase Genes Does not Affect Accumulation of Transgene Nuclear mRNA,” Plant Cell 7 347-358 (1995).
Nishino et al, “Electroantennogram Responses of the American Cockroach to Germacrene D Sex Pheromone Mimic,” J Insect Physiol 23, 415-419 (1977).
Prosser et al “(+)-(10R)-Germacrene A synthase from goldenrod, Solidago canadensis; cDNA isolation, bacterial expression and functional analysis,” Phytochemistry 60, 691-702 (2002).
Schmidt et al “Mechanisms of the Biosynthesis of Sesquiterpene Enantiomers (+)- and (−)-Germacrene D in Solidago canadensis,” Chirality 11:353-362 (1999).
Schmidt et al, “Biosynthese von (+)- und (−)-Germacren D inSolidago canadensis: Isolierung und Charakterisierung zweier enantioselektiver Germacren-D-Synthasen,” Angewandte Chemie 110: 1479-1481 (1998).
Steele et al “Sesquiterpene Synthases from Grand Fir (Abies grandis),” J. Biol. Chem. 273, 2078-2089 (1998).
Steinmetz, A.A. et al., NCBI Entrez Nucleotide, Accession No. AY561842 (online) Mar. 21, 2004 (retrieved Apr. 6, 2004).
Steinmetz, A.A. et al., NCBI Entrez Nucleotide, Accession No. AY561843 (online) Mar. 21, 2004 (retrieved Apr. 6, 2004).
Steliopoulos et al, “Biosynthesis of the sesquiterpene germacrene D inSolidago canadensis: 13C and 2H labeling studies,” Phytochemistry 60: 13-20 (2002).
Trapp & Croteau, “Genomic Organization of Plant Terpene Synthases and Molecular Evolutionary Implications,” Genetics 158, 811-832 (2001).
Van Geldre et al, “Cloning and Molecular Analysis of Two New Sesquiterpene Cyclases from Artemisia annua L,” Plant Sci. 158, 163-171 (2000).
Yoshihara and Hirose “The Sesquiterpenes of Dendropanax trifidus,” Bull Chem Soc Jpn 51:3395-3396 (1978).
Yoshihara et al, “Germacrene D A Key Intermediate of Cadinene Group Compounds and Bourbonenes,” Tetrahedron Letts 27: 2263-2264

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