Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1997-04-25
1998-10-27
Grimes, Eric
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4352543, 4352546, 4353201, 435325, 536 232, C12N 115, C12N 1555, C12N 1563, C12P 2102
Patent
active
058277196
DESCRIPTION:
BRIEF SUMMARY
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a 35 U.S.C. 371 national application of PCT/DK95/00427 filed Oct. 26, 1995 and claims priority under 35 U.S.C. 119 of Danish application 1240/94 filed Oct. 26, 1994, the contents of which are fully incorporated herein by reference.
FIELD OF THE INVENTION
The present invention relates to an enzyme with lipolytic activity, a DNA construct comprising a DNA sequence encoding the enzyme, and a method of producing the enzyme. The invention further relates, inter alia, to enzyme preparations, detergent additives and detergent compositions containing the enzyme, and to the use of the enzyme for a number of industrial and domestic applications.
BACKGROUND OF THE INVENTION
Although numerous lipolytic enzymes (enzymes which catalyze the hydrolysis of lipids, such as triglycerides) have been described and characterized in the literature, there is an continuing need for providing novel lipolytic enzymes, preferably in single-component form, having properties which improve their usefulness in various industrial or domestic applications, such as in detergent compositions for laundry washing or dishwashing, or compositions for hard-surface cleaning, degreasing/defatting and the like.
SUMMARY OF THE INVENTION
The present inventors have now surprisingly succeeded in isolating and characterizing a DNA sequence from a strain of Humicola insolens, which sequence encodes an enzyme exhibiting lipolytic activity, thereby making it possible to prepare a single-component lipolytic enzyme. Lipolytic enzymes of the invention have been found (vide infra) to possess properties which render them very well suited to, inter alia, a number of applications of the above-mentioned types.
Accordingly, in a first aspect the invention relates to a DNA construct comprising a DNA sequence encoding an enzyme exhibiting lipolytic activity, which DNA sequence comprises a lipolytic enzyme, which is obtainable from the plasmid in Saccharomyces cerevisiae DSM 9975, or sequence encoding a lipolytic enzyme, which is obtainable from the plasmid in Saccharomyces cerevisiae DSM 9975, which sequence encoding a lipolytic enzyme, which is obtainable from the plasmid in Saccharomyces cerevisiae DSM 9975, and/or shown in SEQ ID No. 1, and/or the DNA sequence encoding a lipolytic enzyme, which is obtainable from the plasmid in Saccharomyces cerevisiae DSM 9975, and/or by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 1 and/or by the DNA sequence encoding a lipolytic enzyme, which is obtainable from the plasmid in Saccharomyces cerevisiae DSM 9975, and/or antibody raised against a purified lipolytic enzyme which is encoded by the DNA sequence shown in SEQ ID No. 1 and/or by the DNA sequence encoding a lipolytic enzyme, which is obtainable from the plasmid in Saccharomyces cerevisiae DSM 9975, and/or which is derived from Humicola insolens DSM 1800.
It is believed that the DNA sequence shown in SEQ ID NO. 1 is identical to the DNA sequence encoding a lipolytic enzyme, which is obtainable from the plasmid in Saccharomyces cerevisiae DSM 9975.
The DNA sequence shown in SEQ ID NO 1 encodes the mature enzyme with its native signal peptide. Analogously, the amino acid sequence shown in SEQ ID NO 2 constitutes the mature enzyme (amino acids 36-246) with its signal peptide (amino acids 1-35).
It is to be understood that whenever reference is made to "the DNA sequence shown in SEQ ID NO 1 " in connection with the enzyme or DNA construct of the invention (e.g. as used in the above sections a) and b) i)-iv)), said sequence may be the entire sequence shown in SEQ ID NO 1, but is more preferably the part of the sequence which encodes the mature lipolytic enzyme.
Correspondingly, whenever reference is made to "the amino acid sequence shown in SEQ ID NO 2" in connection with the enzyme or DNA construct of the invention, it is to be understood that said sequence may be the entire sequence shown in SEQ ID NO 2, but is more preferably the part of the sequence constituting the mature lipolytic enzym
REFERENCES:
patent: 4810414 (1989-03-01), Huge-Jensen et al.
patent: 5466594 (1995-11-01), Outtrup et al.
patent: 5601978 (1997-02-01), Burczak et al.
Kauppinen Sakari
Kofod Lene Venke
Sandal Thomas
Gregg, Esq. Valeta
Grimes Eric
Novo Nordisk A S
Zelson Esq. Steve T.
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