Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1998-03-18
2000-02-08
Achutamurthy, Ponnathapu
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4352523, 43525411, 4352543, 4352561, 4352563, 4352565, 4352567, 4353201, 435945, 536 232, C12N 924, C12N 120, C12N 114, C12N 100, C07N 2104
Patent
active
060227230
ABSTRACT:
The present invention relates to an enzyme with .beta.-(1-6)-endoglucanase activity encoded by a DNA sequence, which DNA sequence a) comprises the DNA sequence shown in SEQ ID No. 3, or b) comprises an analogue of the DNA sequence shown in SEQ ID No. 3, which i) is homologous with the DNA sequences shown in or SEQ ID No. 3, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 3, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 3, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified .beta.-(1-6)-glucanase shown in SEQ ID No. 4 derived from Trichoderma harzianum, CBS 243.71. The present invention also relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, and the use of the enzyme and enzyme preparation for the degradation or modification of .beta.-glucan containing materials.
REFERENCES:
Mulenga, D.K. et al., Microbios, vol. 10, pp. 143-154 (1994).
Schep, G.P. et al., Biochem. J., vol. 223, pp. 707-714 (1984).
Martin, D.F. et al., Applied and Environ. Microbio., vol. 40, No. 6, pp. 1136-1138 (1980).
Dobourdieu, D. et al., Carbohydrate Research, vol. 144, pp. 277-287 (1985).
Pitson, S.M. et al., Enzyme Microb. Technol., vol. 15, pp. 178-192 (1993).
Andersen Lene Nonboe
Christgau Stephan
Dalb.o slashed.ge Henrik
Kauppinen Markus Sakari
Kofod Lene Venke
Achutamurthy Ponnathapu
Gregg Valeta
Novo Nordisk A S
Slobodyansky Elizabeth
Zelson Steve T.
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