Enzyme with .beta.-(1-6)-endoglucanase activity

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435200, 435277, 435945, C12P 1944, D21C 100, C12N 924, C12N 100

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active

057704066

ABSTRACT:
The present invention relates to an enzyme with .beta.-(1-6)-endoglucanase activity encoded by a DNA sequence, which DNA sequence a) comprises the DNA sequence shown in SEQ ID No. 3, or b) comprises an analogue of the DNA sequence shown in SEQ ID No. 3, which i) is homologous with the DNA sequences shown in or SEQ ID No. 3, and/or ii) hybridizes with the same oligonucleotide probe as the DNA sequence shown in SEQ ID No. 3, and/or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence shown in SEQ ID No. 3, and/or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against a purified .beta.-(1-6)-glucanase shown in SEQ ID No. 4 derived from Trichoderma harzianum, CBS 243.71. The present invention also relates to a DNA construct encoding the enzyme, a method of producing the enzyme, an enzyme preparation containing the enzyme, and the use of the enzyme and enzyme preparation for the degradation or modification of .beta.-glucan containing materials.

REFERENCES:
de la Cruz et al. (1995) J. Bacteriololgy 177, 1864-1871.
Lora et al. (1995) Mol. Gen. Genet. 247, 639-645.
Mulenga, D.K. et al., Microbios, vol. 10, pp. 143-154 (1994).
Schep, G.P. et al., Biochem. J., vol. 223, pp. 707-714 (1984).
Martin, D.F. et al., Applied and Environ. Microbio., vol. 40, No. 6, pp. 1136-1138 (1980).
Dubourdieu, D. et al., Carbohydrate Research, vol. 144, pp. 277-287 (1985).
Pitson, S.M. et al., Enzyme Microb. Technol., vol. 15, pp. 178-192 (1993).

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