Enzyme sensor using immobilized glucokinase

Details Enzyme sensor using immobilized glucokinase Enzyme sensor using immobilized glucokinase

1988-06-24

1990-02-13

Kaplan, G. L.

Chemistry: electrical and wave energy

Apparatus

Electrolytic

357 25, 435 4, 435288, 435817, C12Q 100, C12Q 154

Patent

active

049004239

ABSTRACT:
An enzyme sensor comprising an enzyme acting specifically on a substrate and a transducer for converting the quantitative change of a substance or heat which is produced or consumed during an enzyme reaction to an electrical signal, wherein the enzyme is glucokinase is disclosed. By the enzyme sensor of the present invention, an accurate determination of an amount of glucose in a sample is possible as well as an accurate determination of adenosine-5'-triphosphate (ATP). A response time of the sensor is almost constant after a long-term use and a decrease of the detecting ratio is very small.

REFERENCES:
patent: 4020830 (1977-05-01), Johnson et al.
patent: 4657854 (1987-04-01), Wegfahrt
M. Y. Kamel et al., Analytical Biochemistry, 18, 270-273 (1967).
Patent Abstracts of Japan, vol. 10, No. 308 10-21-86, Mitsubishi Electric Corp. abst. & JPA 61122560.
Chemical Abstracts, vol. 95, p. 304 #2947z 1981, "Determination of Adenosine Triphosphate with an Enzyme Electrode", J. Kulys et al. Columbus, OH.

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