Enzyme quantitation wicking assay

Details Enzyme quantitation wicking assay Enzyme quantitation wicking assay

1991-10-15

1993-02-23

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 76, 435 792, 435 18, 435970, 435975, 422 56, 422 57, 436518, 436514, 436531, 436538, G01N 33535, G01N 33558, G01N 33537

Patent

active

051889382

ABSTRACT:
Diagnostic assays are provided comprising complementary enzyme fragments of .beta.-galactosidase, where one of the fragments is bound to a support and the other fragment is conjugated to an immunologically cross-reactive epitope of the analyte or complementary to an analyte receptor. Binding to the receptor allows for discrimination between complexed enzyme fragment conjugate and uncomplexed enzyme fragment conjugate. The assay medium is then allowed to wick onto a support to which the complementary fragment is substantially uniformly bound in the detection region. The support is then developed, where color formation from the substrate in the detection region is used as a measure of the presence of analyte in the sample.

REFERENCES:
patent: 4708929 (1987-11-01), Henderson
patent: 4740468 (1988-04-01), Weng et al.
patent: 4818677 (1989-04-01), Hay-Kaufman et al.
patent: 4937188 (1990-06-01), Giese et al.
patent: 5037735 (1991-08-01), Khanna et al.
Langley, et al., ".beta.-Galactosidase .alpha. Complementation Properties of the Complemented Enzyme and Mechanism of the Complementation Reaction" in Biochemistry (1976) 15(22):4866-4875.

Affiliated with

Also associated with

Rating

Enzyme quantitation wicking assay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzyme quantitation wicking assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme quantitation wicking assay will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2204111