Enzyme-protein complex

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S007600, C435S007940, C435S964000

Reexamination Certificate

active

10608025

ABSTRACT:
A complex of enzyme, protein and carrier comprising two or more molecules of an enzyme conjugated through an amino group or other group to a carrier such as polylysine, and a protein (for example, antibody) with a specific binding potency to other substance(s) (for example, antigen) conjugated to at least one of said two or more molecules of the enzyme. This complex enables accurate assay of a trace amounts of a substance at a high sensitivity.

REFERENCES:
patent: 5019498 (1991-05-01), Chichibu
patent: 5053520 (1991-10-01), Bieniarz et al.
patent: 5057313 (1991-10-01), Shih et al.
patent: 5084560 (1992-01-01), Hellstrom et al.
patent: 5658741 (1997-08-01), Bolton et al.
patent: 5763158 (1998-06-01), Bohannon
patent: 5833924 (1998-11-01), McClintock et al.
patent: 6252053 (2001-06-01), Ohbayashi et al.
patent: 0 122 028 (1984-10-01), None
patent: 0 123 300 (1984-10-01), None
patent: 0 151 492 (1985-08-01), None
patent: 0 155 224 (1985-09-01), None
patent: 0 269 451 (1988-06-01), None
patent: 0 992 794 (2000-04-01), None
patent: 59-220199 (1984-12-01), None
patent: 59-224564 (1984-12-01), None
patent: 63-503138 (1988-11-01), None
patent: 3-158758 (1991-07-01), None
patent: 6-509167 (1994-10-01), None
patent: 7-216000 (1995-08-01), None
patent: 10-084959 (1998-04-01), None
patent: WO 87/05031 (1987-08-01), None
patent: WO 93/01498 (1993-01-01), None
patent: WO 95/24631 (1995-09-01), None
patent: WO 97/14028 (1997-04-01), None
Physical Properties of Dextran- Internet update information Jun. 28, 2006.
Inoue S., et al., “A micro-scale method for the conjugation of affinity-purified Fab1to beta-D-galactosidase fromEscherichia coli”, J. Biochem, Nov. 1985; 98(5):1387-94., 1 page.
Setsuko Ishikawa, et al., “Sensitive Enzyme Immunoassay of Antibodies to HIV-1 p17 Antigen Using Indirectly Immobilized Recombinant p17 for Diagnosis of HIV-1 Infection”, Journal of Clinical Laboratory Analysis, 12: 1998, pp. 343-350.
Derwent Publications, AN 1990-286254, JP 02 201162, Aug. 9, 1990.
Derwent Publications, AN 1991-196013, JP 03 089165, Apr. 15, 1991.
Derwent Publications, AN 1991-153208, JP 03 089164, Apr. 15, 1991.
Derwent Publications, AN 1990-113509, JP 02 066459, Mar. 6, 1990.
Derwent Publications, AN 1986-281802, JP 61-205863, Sep. 12, 1986.
Derwent Publications, AN 1984-034233, JP 58-225028, Dec. 27, 1983.
Pierce Catalog Handbook, 13 pages, “Technical Section,” 1994-1995.
A.H. Blair, et al., Journal of Immunological Methods, vol. 59, pp. 129-143, “Linkage of Cytotoxic Agents to Immunoglobulins,” 1983.
R.P. Haugland, Methods in Molecular Biology, vol. 45, pp. 235-243, “Coupling of Monoclonal Antibodies With Enzymes,” 1995.
E. Ishikawa, et al., Journal of Immunoassay, vol. 4, No. 3, pp. 209-327, “Enzyme-Labeling of Antibodies and Their Fragments for Enzyme Immunoassay and Immunohistochemical Staining,” 1983.
S. Hashida, et al., Journal of Applied Biochemistry, vol. 6, pp. 56-63, “More Useful Maleimide Compounds of the Conjugation of FAB' to Horseradish Peroxidase Through Thiol Groups in the Hinge,” 1984.
P.K. Nakane, et al., The Journal of Histochemistry and Cytochemistry, vol. 14, No. 12, pp. 929-931, “Enzyme-Labeled Antibodies: Preparation and Application for the Localization of Antigens,” 1967.
P.K. Nakane, et al., The Journal of Histochemistry and Cytochemistry, vol. 22, No. 12, pp. 1084-1091, “Peroxidase-Labeled Antibody a New Method of Conjugation,” 1974.
Z.-R. Shi, et al., The Journal of Histochemistry and Cytochemistry, vol. 36, No. 3, pp. 317-322, “A Comparison of Three Immunoperoxidase Techniques for Antigen Detection in Colorectal Carcinoma Tissues,” 1988.
R. Giorno, Diagnostic Immunology, vol. 2, pp. 161-166, “A Comparison of Two Immunoperoxidase Staining Methods Based on the Avidin-Biotin Interaction,” 1984.
K. Fujiwara, et al., Journal of Immunological Methods, vol. 110, pp. 47-53, “The Use ofN-[β-(4-Diazophenyl)Ethyl]Maleimide as a Coupling Agent in the Preparation of Enzyme-Antibody Conjugates”, 1988.
C. A. Rowe, et al., Anal. Chem., vol. 71, pp. 3846-3852, “Array Biosensor for Simultaneous Indentification of Bacterial, Viral, and Protein Analytes”, 1999.
S. S. Ghosh, et al., Bioconjugate Chem., vol. 1, pp. 71-76, “Use of Maleimide-Thiol Coupling Chemistry for Efficient Syntheses of Oligonucleotide-Enzyme Conjugate Hybridization Probes”, 1990.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Enzyme-protein complex does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzyme-protein complex, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme-protein complex will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3869546

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.