Enzyme production and purification

Details Enzyme production and purification Enzyme production and purification

1981-06-26

1983-08-16

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435813, 435818, 435101, 435103, C12N 910, C12P 1904, C12P 1908

Patent

active

043992218

ABSTRACT:
There is described a process for the production of a polysaccharide-producing enzyme, which comprises fermenting an appropriate enzyme producing organism in aqueous sucrose, characterized in that the sucrose concentration is maintained at between 1 and 10 g/l throughout all but the last part of the fermentation and that the dissolved oxygen content of the fermentation is controlled.
There is also described a method for the purification of a polysaccharide producing enzyme, which comprises absorbing a mixture containing the enzyme on an anionic resin and removing the enzyme from the resin with a sugar containing eluant, and a novel highly purified form of polysaccharide producing enzyme.

REFERENCES:
patent: 2673828 (1954-03-01), Koepsell et al.
patent: 2686147 (1954-08-01), Tsuchiya et al.
patent: 3060103 (1962-10-01), Kaufmann et al.
patent: 4309505 (1982-01-01), Smith et al.

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