Enzyme preparation for serum cardiac glycoside assay

Details Enzyme preparation for serum cardiac glycoside assay Enzyme preparation for serum cardiac glycoside assay

1972-08-11

1976-05-25

Naff, David M.

Textiles: fiber preparation

195 68, 195 99, 1951035R, 1951037, G01N 3114, C07G 702

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active

039590771

ABSTRACT:
A method of assaying serum cardiac glycoside concentrations employing the principle of enzymatic isotope displacement from an enzymatically produced binding enzyme, a serum extract, and a labeled or isotopic cardiac glycoside. The serum extract is prepared by extracting the serum with a halogenated alkane, and partitioning the extract between a buffered solution and an aromatic hydrocarbon having a molecular weight greater than 80. The binding enzyme is made from an enzymatic preparation of whole brain by reacting the preparation with calcium, sodium, magnesium, and ATP.

REFERENCES:
brooker et al., Settum Digoxin Levels in Toxic and Nontoxic Patients by Enzymatic Isotope Displacement, Clinical Research, Vol. 18, Apr. 1970 (p. 299).
Bonting et al., Studies on Sodium-Posassium-Activated Adenosinetriphosphatase, Archives of Biochemistry and Biophysics Vol. 98, 1962 (pp. 413-419).
Skoll, J. C., The Influence of Some Cations on an Adenosine Triphosphatase from Peripheral Nerves, Biochimica et Biophysica Acta, Vol. 23, 1957 (pp. 394-401).
Dixon, et al., Enzymes, 2nd Ed., Academic Press Inc., N.Y., 1964 (pp. 148- 150 & 423).

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