Enzyme of use in chitosan hydrolysis

Details Enzyme of use in chitosan hydrolysis Enzyme of use in chitosan hydrolysis

1992-12-14

1996-01-09

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435101, 435209, 530 232, C12N 942, C12N 1556, C12P 1926, C12P 1904

Patent

active

054828430

ABSTRACT:
The present invention relates to a naturally occuring, isolated and characterized microorganism producing hydrolase enzyme, specifically, chitosanase; chitosanase isolated thereof; the nucleic acid sequence of the portion of the gene encoding this chitosanase; complete amino acid sequence of this chitosanase; hybrid plasmids containing the related gene; host microorganisms transformed with the plasmids; recombinant microorganisms overexpressing the enzyme; and to enzymatic treatment of chitosan with the aforementioned enzyme resulting in molecular weight decrease, viscosity decrease and increase of solubility of chitosan in water.

REFERENCES:
D. Fink et al. "Cloning and Expression in Streptomyces . . . " Biotechnology Letters. 13(12) 845-850 (Dec. 1991).
I. Boucher et al. "Purification and Characterization of a . . . " Applied Microbiol. and Biotechnol. 38(2) 188-193 (Nov. 1992).
Denis, F., et al., (1992). Gene 111: 115.
Denis, F., et al., (1991). FEMS Microbiol. Lett. 81: 261-264.
Neugebauer, E., et al. (1991). Arch. Microbiol. 156: 192-197.
Pelletier, A., et al. (1990). Appl. Environ. Microbiol. 56(4): 844-848.
Price, J. S., et al. (1975). J. Bacteriol. 124(3): 1574-1585.
Sakai, K., et al. (1991). Biochim. Biophys. Acta 1079: 65-72.

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