Enzyme of hydrolytic action, enzymatic preparation, method for p

Details Enzyme of hydrolytic action, enzymatic preparation, method for p Enzyme of hydrolytic action, enzymatic preparation, method for p

1976-11-08

1978-01-03

Shapiro, Lionel M.

Textiles: fiber preparation

195 66R, 195 65, 424 94, C12D 1310, A61K 3754

Patent

active

040665038

ABSTRACT:
An enzyme of hydrolytic action, according to the invention, is a neutral protease having the molecular weight of about 37,000, the isoelectric point at pH 8.8, the optimum activity at 40.degree. - 45.degree. C, the stability range in aqueous solutions at pH 6.8 - 8.0, quickly inactivating in said solutions at temperatures above 50.degree. C.
The enzymatic preparation containing the enzyme of hydrolytic action in an amount of 50 - 500 PU/g and the metabolites of the strains Streptomyces hygroscopicus.
A method for preparing the hydrolytic enzyme, in which the strain Streptomyces hygroscopicus is cultivated on media containing assimilable sources of carbon and nitrogen, inorganic mineral salts, in deep aerobic conditions, with accumulation of the enzyme of hydrolytic action and the metabolites of the said strains in the culture fluid, followed by subsequent isolation of the enzyme.
A medicinal preparation for treating respiratory diseases, purulent wounds and thermal burns, for treating and preventing empyema of the pleura, containing the enzyme of hydrolytic action in the quantity of 1 - 50 PU/mg, inactive protein and mineral salts.

REFERENCES:
patent: 3875006 (1975-04-01), Belloc et al.

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