Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1984-08-20
1987-04-21
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
204403, 435176, 435180, 435288, 435817, C12N 1104, C12N 1114, C12N 1108, C12M 140
Patent
active
046596658
ABSTRACT:
A film or membrane containing a biologically active protein such as an enzyme is prepared from a polymer substituted with acyl hydrazide groups. Preferably, the polymer is an acrylamide/methacrylamide copolymer in the ratio of about 70/30, and the film or membrane is about 30-100 microns thick. The film or membrane is prepared on an electrode and crosslinked to produce an enzyme electrode.
REFERENCES:
patent: 3542662 (1970-11-01), Hicks et al.
Freeman, Gel Entrapment of Whole Cells and Enzymes in Crosslinked, Prepolymerized Polyacrylamide Hydrazide, Enzyme Engineering, 7, vol. 434.
Freeman et al., New Enzyme Membrane for Enzyme Electrodes, Analytical Chemistry, 1986.
Durand et al., An Enzyme Electrode for Acetylcholine, Biochimica et Biophysica Acta, 527, (1978), 277-281.
Freeman et al., Immobilization of Microbial Cells in Crosslinked, Prepolymerized, Linear Polyacrylamide Gels: Antibiotic Production by Immobilized Streptomyces Clavuligerus Cells, Biotechnology and Bioengineering, vol. XXIII, pp. 2747-2759 (1981).
Nilsson et al., Determination of Glucose, Urea and Penicillin Using Enzyme-pH-Electrodes, Biochimica et Biophysica Acta, 320, (1973), 529-534.
Guilbault et al, Improved Urea Electrode, Analytical Chemistry, vol. 45, No. 2, Feb. 1973.
Bowers et al, Immobilized Enzymes in Analytical Chemistry, Advanced Biochem. Eng. 15, 89-129, 1980.
Freeman Amihay
Tor Ruth
Naff David M.
Ramot University Authority for Applied Research and Industrial
LandOfFree
Enzyme membranes for electrodes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Enzyme membranes for electrodes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme membranes for electrodes will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-751469