Enzyme-linked immunosorbent assay (ELISA) for determining anti-b

Details Enzyme-linked immunosorbent assay (ELISA) for determining anti-b Enzyme-linked immunosorbent assay (ELISA) for determining anti-b

1984-12-28

1988-08-16

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435810, 436511, 436548, 436809, 436825, C12Q 170, G01N 33571, G01N 33577

Patent

active

047644591

ABSTRACT:
A method and test kit for the serological diagnosis of human infection by herpes sipmlex virus type 1 (HSV-1) and herpes simplex virus type 2 (HSV-2) using immunoaffinity purified virus-coded glycoproteins as target antigens. A preferred embodiment of the method employs a variation of the enzyme-linked immunosorbent assay (ELISA) whereby monoclonal antibodies are used to purify target antigens, and test sera are absorbed with virus-infected cell extracts to remove intertypic cross-reacting antibodies.

REFERENCES:
patent: 4430437 (1984-02-01), Hampar et al.
patent: 4446232 (1984-05-01), Liotta
patent: 4450231 (1984-05-01), Ozkan
patent: 4535057 (1985-08-01), Dreesman et al.
patent: 4572896 (1986-02-01), Hampar et al.
Zweig et al., "J. of Virology" vol. 47, No. 1, (Jul. 1983), pp. 185-192.
Showalter et al., "Infec. and Immun." vol. 34, No. 3, (Dec. 1981), pp. 684-692.

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