Enzyme linked immunoassay with stabilized polymer saccharide enz

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435180, 435181, 435182, 435183, 435188, 530200, 530201, 530202, 530203, 530204, 530205, 530206, 530207, G01N 3353, C12N 1106, C12N 1108, C12N 996, C12N 900

Patent

active

056911548

ABSTRACT:
An improvement in enzyme linked immunoassays is disclosed wherein the enzyme is in the form of a water soluble polymer saccharide conjugate which is stable in hostile environments. The conjugate comprises the enzyme which is linked to the polymer at multiple points through saccharide linker groups.

REFERENCES:
patent: 3625827 (1971-12-01), Wildi et al.
patent: 3806417 (1974-04-01), Beaucamp et al.
patent: 4038140 (1977-07-01), Jaworek et al.
patent: 4081329 (1978-03-01), Jaworek et al.
patent: 4119589 (1978-10-01), Horn et al.
patent: 4182695 (1980-01-01), Horn et al.
patent: 4652524 (1987-03-01), Modrovich et al.
patent: 4673707 (1987-06-01), Tsai et al.
patent: 4711840 (1987-12-01), Nowinski et al.
patent: 4845035 (1989-07-01), Fanta
patent: 5041292 (1991-08-01), Feijen
Stryer, "Biochemistry," (1988), Freeman Co., N.Y., pp. 302-303.
Biotechnology Letters, Vol. 6, No. 12, issued 1964; K. Takahashi et al., "Polyethylene Glycol-Modified Enzymes Trap Water On Their Surface and Exert Enzymic Activity in Organic Solvents," pp. 765-770.
Die Angewandte Makromolekulare Chemie, Vol. 76/77, issued 1979, U. Seitz et al., "Neu Reaktive Mikrogele als Trager fur Enzyme," pp. 319-327.
Trends in Biochem. Sci., vol. 3, issued 1978, J.J. Marshall, "Manipulation of the Properties of Enzymes by Covalent Attachment of Carbohydrate," pp. 79-83.
Cell Surface Carb. and Biol. Recogn., issued 1978, G.R. Gray et al., "Proteins Containing Reductively Aminated Disaccharides: Chemical and Immunochemical Characterization," pp. 583-594.
Trends in Biochemical Tech., Vol. 9, No. 11, issued Nov. 1991, B. Mattiasson et al., "Tailoring the Microenvironment of Enzymes in Water-Poor Systems," pp. 394-398.
K. Takemoto et al., "Functional Monomers and Polymers, Procedures, Synthesis, Applications, "published 1987 by Marcel Dekker (N.Y.), pp. 461-503.
Makromol. Chem., vol. 188, issued 1987, J. Klein, "Synthesis of Some Poly (Vinylsaccharide)s of the Amide Type and Investigation of Their Properties," pp. 1217-1232.
J. Polymer Sci., PART A-1, Vol. 6, issued 1968, Y. Iwakura et al., "Preparation of Polymers Containing Sugar Residues," pp. 1625-1632.
Enzyme Microb. Technol., vol. 13, issued Jul. 1991, H. Kise et al., "Immobilization of Proteases to Porous Chitosan Beads and Their Catalysis for Ester and Peptide Synthesis in Organic Solvents," pp. 584-588.
Enzyme Microb. Technol., vol. 14, issued Feb. 1992, S.A. Khan et al., "Polyethylene Glycol-Modified Subtilisin Forms Microparticulate Suspensions in Organic Solvents," pp. 96-100.
Enzyme Microb. Technol., vol. 14, issued Feb. 1992, H.F. Gaertner et al., "Increased Activity and Stability of Poly(Ethylene Glycol) -Modified Trypsin," pp. 150-155.
J. Am. Chem. Soc., vol. 114, issued Jan. 1992, P. Wang et al., "New Carbohydrate-Based Materials for the Stabilization of Proteins," pp. 378-380.
Chem Eng. News, issued 13 Jan. 1992, S. Borman, "Immobilized Enzymes Act in Organic Solvents," pp. 5-6.
Makromol. Chem. Rapid Commun., vol. 6, issued 1985, J. Klein et al., "Emulsion Polymerization of Poly (Meth-acryloylglucose)," pp. 675-678.
Makromol. Chem. Rapid Commun. vol. 7, issued 1986, A. Kobayashi et al. "Enhanced Adhesion and Survival Efficiency of Liver Cells in Culture Dishes Coated with a Lactose-Carrying Styrene Homopolymer," pp. 645-650.
O'Neil et al., Biotechnol. and Bioeng., XIII, 319-322 (1971).
Patel et al., Biopolymers, 5, 577-582 (1967).
Patel et al, Biopolymers, 5, 583-584 (1967).
Robinson et al., Biochim. Biophys. Acta, 242, 659-661 (1971).
Weibel et al, Biochem. J., 125, 801-807 (1971).
Yakunitskaya et al., Preparation of Conjugates of Subtilisin BPN' and Dextrans, 174-178 (1980).
Axen et al., Biopolymers, 9, 401-413 (1970).
Barker et al., Carbohyd. Res., 20, 1-7 (1971).
Davey et al., Enzyme Microb. Technol.,11, 657-661, (1989).
Fisher et al, Macrolide Antibiotics 553-606 (1984).
Kilbanov, Chemtech, 354-359 (1986).
Luthra et al., J. Chem. Soc. Perkin Trans. II., 1575-1578 (1987).
Manecke et al. Reactive Polymers, 3, 251-260 (1985).
Mitz et al., Nature, 169, 576-577 (1961).
Mozhaev et al., Enzyme Microb. Technol., 6, 50-59 (1984).
Russell et al., J. Biol. Chem., 263, 11624-11626 (1988).
Russell et al., Biochem. Soc. Trans., 17, 1145, (1989).
Suen et al., Makromol. Chem., 186, 255-260 (1985).
Torchilin et al., Biochimica et Biophysica Acta, 522, 277-283 (1978).
Wong et al., J. Am. Chem. Soc., 945-953 112, (1990).
Wykes et al., Biochim. Biophys. Acta, 250, 522-529 (1971).
Zaks et al., Proc. Natl. Acad. Sci., USA, 82, 3192-3196 (1985).
Zaks et al., Science, 224, 1249-1251 (1984).
Bird et al., Chemistry and Industry, 1331-1332 (1960).
Black et al., J. Chem. Soc., 4433-4439 (1963).
Black et al., Chemistry and Industry, 1624 (1962).
Haworth et al., J. Chem. Soc., 488-491 (1946).
Imoto et al., Institute of Polytechnics, 210-211 (1962).
Kimura et al., Institute of Polytechnics, 232-236 (1962).
Kimura et al., Institute of Polytechnics, 155-160 (1961).
Klein et al., Makromol. Chem., Rapid Communication, 7, 621-625 (1986).
Nichols, Jr. et al., J. Am. Chem. Soc., 2020-2022 (1946).
Nichols, Jr. et al., J. Am. Chem. Soc., 46-49 (1945).
Nichols, Jr. et al., J. Am. Chem. Soc., 1625-1627 (1944).
Otey et al., Ind. Eng. Chem. Prod. Res. Develop., 11, 70-73 (1972).
Overberger et al., J. of Pol. Sci. 7, 1011-1022, (1969).
Treadway et al., J. Am. Chem. Soc., 1038-1039 (1945).
Whistler et al., J. Org. Chem.,2961-2962 (1962).
Barbas et al., J. Am. Chem. Soc. 110, 5162-5166 (1988).
Jakubke et al., Angew. Chem. Int. Ed. Engl. 24, 85-93 (1985).
Kasche, Proteolytic Enzymes, 125-143 (1989).
Kitaguchi et al., J. Am. Chem. Soc., 111, 9272-9273 (1989).
Kuhl et al., Tetrahedron Let. 21, 893-896 (1980).
Luthi et al., J. Am. Chem. Soc., 106, 7285-7286 (1984).
Margolin et al., J. Am. Chem. Soc., 109, 3802-3804 (1987).
Margolin et al., J. Am. Chem. Soc., 109, 7885-7885 (1987).
Morihara et al., Biochem. J., 163, 531-542 (1977).
Pugh, "Enzymes as Catalysts in Organic Synthesis," 217-232 (1986).
West et al., J. Chem. Soc., Chem. Commun., 417-418 (1986).
Gray, "Preparation of Antigens," 155-160.
Lonngren et al., "Preparation of Antigens," 160-162.
Smith et al., "Preparation of Antigens," 169-171.
Zopf et al., "Preparation of Antigens," 163-169.
Zopf et al., "Preparation of Antigens," 171-175.
Cramer, Methods in Carbohydrate Chemistry, 1, 242-246 (1962).
Mrozik et al., J. Med. Chem., 25, 658-663 (1982).
Richardson, Methods in Carbohydrate Chemistry 6, 218-224 (1972).
Zaborsky, "Immobilized Enzymes", 49-60, 149-165 (1973).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Enzyme linked immunoassay with stabilized polymer saccharide enz does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzyme linked immunoassay with stabilized polymer saccharide enz, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme linked immunoassay with stabilized polymer saccharide enz will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2105906

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.