Enzyme-linked immunoassay for measurement of cyclosporin a level

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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436525, 436518, 436541, 436178, 436815, 435962, C12Q 100, G01N 33543, G01N 33553, G01N 33538

Patent

active

051513480

ABSTRACT:
An enzyme-linked immunoassay for the determination of cyclosporin A levels in whole blood samples is provided based on the utilization of .beta.-D-galactosidase as an enzyme label and chlorophenol red-.beta.-D-galactopyranoside or resorufin-.beta.-D-galactopyranoside as a .beta.-D-galactosidase substrate.

REFERENCES:
patent: 4235960 (1980-11-01), Sasse et al.
patent: 4668622 (1987-05-01), Kuhr et al.
patent: 4727035 (1988-02-01), Mahoney
Tijssen, P. Practice and Theory of Enzyme Immuno Assays, Elsevier, Amsterdam, pp. 188-191, 246-249 (1985).
Mahler et al., Basic Biological Chemistry, FIG. 15-4 (1968).
Kaplan et al., Clinical Chemistry, p. 631.
Quesniaux et al., "Potential of Monoclonal antibodies to Improve Therapeutic Monitoring of Cyclosporine," Clin Chem 33:32-37 (1987).
Lensmeyer et al., Transplantation, 42(4): 372-376 (1986).
Foxwell et al., Biochimica et Biophysica Acta, 938:447-445 (1988).
Quesniaux et al., Eur. J. Immunol., 17: 1359-1365 (1987).
Agarwal et al., Clin. Chem., 33(4): 481-485 (1987).
Ball et al., Clin. Chem., 34(2): 257-260 (1988).
Sandimmun.RTM.-Kit Product Insert Sheet, Sandoz Ltd.
TDx.RTM. Cyclosporine and Metabolites Whole Blood Assay Information, Abbott Laboratories (1988).
Cyclo-Trac.RTM. SP Whole Blood Radioimmunoassay for Cyclosporine Procuct Insert Sheet, Incstar.
Beresini, M., EMIT.RTM. Cyclosporine Assay for Whole Blood Samples on the COBAS MIRA Analyzer, Poster Presentation at the AACC/ICCC Meeting (1990).

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