Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-11-24
2000-12-12
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 75, 435 79, 435 791, 435 792, 435 6, 435975, 435966, 435968, 436501, G01N 3353, G01N 33566
Patent
active
06159699&
ABSTRACT:
A method is provided for determining in a medium the presence or amount of an analyte which is capable of binding to a ligand partner to form a ligand complex, which method comprises: (a) contacting the medium with either: (i) a ligand partner conjugated with an enzyme being capable of catalysing a reaction, or one or more reactions in a cascade thereof, to produce hydrogen peroxide, or (ii) a ligand partner and either a competing analyte or an analog of said analyte, a competing analyte or analyte analog being capable of forming a ligand complex with the ligand partner and the competing analyte or analyte analog being conjugated with an enzyme capable of catalysing a reaction, or one or more reactions in a cascade thereof, to produce hydrogen peroxide, (b) optionally separating complexed and uncomplexed enzyme conjugates, (c) causing or allowing the reaction or cascade of reactions to occur to produce hydrogen peroxide by contacting the complexed or uncomplexed enzyme conjugate with a corresponding enzyme substrate; (d) contacting hydrogen peroxide with a substance capable of exhibiting chemiluminescence in the presence of hydrogen peroxide selected from the group consisting of acridinium compounds and analogs thereof having a conjugate base with a pKa less then 9 to generate a chemiluminescent reaction, and (e) detecting the occurrence of said chemiluminescent reaction to determine the presence or amount of the analyte. Kits for use in connection with the method are also disclosed.
Brown Richard Charles
Weeks Ian
Chin Christopher L.
Do Pensee T.
Molecular Light Technology Limited
LandOfFree
Enzyme linked chemiluminescent assay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Enzyme linked chemiluminescent assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme linked chemiluminescent assay will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-215438