Enzyme-labeled antibody reagent with polyalkyleneglycol linking

Details Enzyme-labeled antibody reagent with polyalkyleneglycol linking Enzyme-labeled antibody reagent with polyalkyleneglycol linking

1986-07-24

1989-03-07

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 14, 435188, 436547, 436548, 436512, 530390, 530391, 530408, 530409, 548521, G01N 33535

Patent

active

048106380

ABSTRACT:
Enzyme-labeled antibody reagents wherein the enzyme and an antibody reagent, e.g., whole polyclonal or monoclonal antibody or a fragment thereof, are covalently linked through a bis-maleimidopolyalkyleneglycol bridge group. The reagents are useful in immunoassay and other methods for detecting an antigen or hapten that can be bound by the antibody reagent. The conjugates are highly stable and water soluble, and are characterized by a high degree of immunoreactivity and enzyme activity.

REFERENCES:
patent: 4101380 (1978-07-01), Rubinstein
Ishikawa Journal of Immunoassay 4(3), pp. 209-227, (1983).

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