Enzyme-kinetic determination of the concentration of a substrate

Details Enzyme-kinetic determination of the concentration of a substrate Enzyme-kinetic determination of the concentration of a substrate

1974-10-02

1976-08-31

Naff, David M.

Textiles: fiber preparation

195 63, 195 99, C12K 104, G01N 3114, G01N 3316

Patent

active

039779440

ABSTRACT:
Enzyme-kinetic determination of the concentration of an enzyme substrate which is acted on during an enzymatic reaction is carried out by adding at least one competitive inhibitor to an enzymatic reaction medium to bring the ratio of the substrate concentration C.sub.3 to the apparent Michaelis constant K'.sub.M to 0.2:1 or less, homogenizing the medium and determining the rate change of the enzymatic reaction at certain intervals of time.

REFERENCES:
patent: 3627645 (1971-12-01), Grassetti
Zollmer et al, "Heparin Determination With Ribonuclease", Methods of Enzymatic Analysis H. U. Bergmeyer, 1965, pp. 79-83.

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