Enzyme isolated from a Bifidobacterium

Details Enzyme isolated from a Bifidobacterium Enzyme isolated from a Bifidobacterium

2006-07-25

2006-07-25

Slobodyansky, Elizabeth (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S193000, C435S201000, C426S582000, C426S583000

Reexamination Certificate

active

07081355

ABSTRACT:
The present invention concerns a new β-galactosidase with transgalactosylating activity isolated fromBifidobacterium bifidumand a truncated enzyme where the C-terminal end of the β-galactosidase protein has been deleted, resulting in an enzyme with a higher transgalactosylating activity than hydrolase activity. When lactose is used as a substrate, galacto-oligosaccharides are products of the transgalactosylase activity. Galacto-oligosaccharides enhance growth of health-promotingBifidobacteriumthat may be used in a number of applications in the dairy industry.

REFERENCES:
patent: 5939289 (1999-08-01), Ertesvag et al.
Jorgensen et al (Dec. 2001) Applied Microbiology and Biotechnology, vol. 57, pp. 647-652.
Moller et al. (May 2001) Applied Environmental Microbiology, vol. 67 (5), pp. 2276-2283.
V. Dumortier et al. “Purification and properties of a β-d-galactosidase fromBifidobacterium bifidumexhibiting and transgalactosylation reaction”,Biotechnol. Appl. Biochem., vol. 19, pp. 341-354 (1994).
R.E. Huber et al., “A quantitation of the factors which affect the hydrolase and transgalactosylase activities of β-galactosidase (E. coli) on lactose”,Biochemistry, vol. 15, No. 9, pp. 1994-2001 (1976).
M. Nakao et al., “Purification and characterization of a thermostable β-galactosidase with high transgalactosylation activity fromSaccharopolyspora rectivirgula”, Appl. Microbiol Biotechnol., vol. 40, pp. 657-663 (1994).
N. Onishi et al., “Purification and properties of a novel thermostable galacto-oligosaccharide-producing β-galactosidase fromSterigmatomyces elviaeCBS8119”,Applied and Environmental Microbiology, vol. 61, No. 11, pp. 4026-4030 (Nov. 1995).
M.V.W. Wijnands et al., “A comparison of the effects of dietary cellulose and fermentable galacto-oligsaccharide, in a rat model of colorectal carcinogenesis: fermentable fibre confers greater protection then non-fermentable fibre in both high and low fat backgrounds,”,Carcinogenesis, vol. 20, No. 4, pp. 651-656 (1999).

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