Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose
Patent
1993-05-25
1996-06-18
Beisner, William H.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving glucose or galactose
435 15, 435 791, 435942, C12Q 154, C12Q 148
Patent
active
055276873
ABSTRACT:
A method for screening for sterol biosynthesis inhibitors of potential use as fungicides or antihypercholesterolemic agents identifies agents by the induction of lanosterol 14-.alpha.-demethylase, an enzyme in the biosynthetic pathway of ergosterol and cholesterol, in cultures containing the agents. In one screening test, test samples are incubated in a culture of a Saccharomyces cerevisiae strain sensitive to ergosterol biosynthesis and containing a gene fusion of a lanosterol 14-.alpha.-demethylase clone with a gene for bacterial .beta.-galactosidase. After incubation of the culture, an increase in lancsterol 14-.alpha.-demethylase activity is determined indirectly by measuring .beta.-galactosidase activity. The culture media contains a chromogenic substrate of .beta.-galactosidase such as orthonitrophenyl-.beta.-D-galactoside or 5-bromo-4-chloro-3-indoyl-.beta.-D-galactoside, so that active samples are identified by the production of colored product. For comparison purposes, screening tests may employ a lanosterol 14-.alpha.-demethylase inhibitor such as dinaconazole as a positive control.
REFERENCES:
patent: 5236955 (1993-08-01), Gordon
Blaloch R., Inhibition of Sterol .fwdarw..sup.8 >.increment..sup.7 Isomerase . . . Phytochemistry vol. 26 No. 3 pp. 663-668 1987.
Balliano G., Inhibition of Sterol Biosynthesis . . . Biochim et Biophys Acta 959 1988 pp. 9-19.
Balliano G., Differential Inhibition of Fungal . . . Febs Letters vol. 320 #3 pp. 203-246 1993.
Ausubel, F. M., et al., eds., Current Protocols in Molecular Biology, John Wiley, New York, 1989, Units 13.4, 13.6, 13.7 and 16.4.1.
Basson, M. E., et al., Genetics 117:645-655 (1987).
Casadaban, M. J. et al., Methods Enzymol. 100: 293-308 (1983).
Gaber, R. F., et al., Mol. Cell. Biol. 9: 3447-3456 (1989).
Guarente, L., Methods in Enz. 101: 181-191 (1983).
Hinnebusch, A. G., Micro. Rev. 52: 248-273 (1988).
Ikeura, R., et al., J. Antibiotics 41: 1148-1150.
Kalb, V. F., et al., Gene 45: 237-245 (1986).
Kalb, V. F., et al., DNA 6: 529-537 (1987).
Turi, T. G., and Loper, J. C., J. Biol. Chem. 267: 2046-2056 (1992).
Kirsch Donald R.
Lai Margaret H. K.
American Cyanamid Company
Beisner William H.
Gitomer Ralph
Webster Darryl L.
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