Enzyme/immunofluorescent assay for autoantibodies

Details Enzyme/immunofluorescent assay for autoantibodies Enzyme/immunofluorescent assay for autoantibodies

1982-05-14

1984-12-11

Hazel, Blondel

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 28, 436506, 436508, 436509, 436519, G01N 3354, C12Q 128

Patent

active

044878303

ABSTRACT:
A method for the determination of autoantibody in a test sample comprises contacting a substrate for the autoantibody with sample; treating the contacted substrate with labeled antihuman antibody selected from (1) a mixture comprising enzyme labeled antihuman antibody and fluorescent labeled antihuman antibody, and (2) antihuman antibody labeled with an enzyme and a fluorescent label; determining the enzyme activity of the treated substrate; and determining the immunofluorescent patterns in substrates exhibiting enzyme activity. The method is useful for the rapid screening and characterization of autoantibodies.

REFERENCES:
patent: 4144031 (1979-03-01), Acevedo et al.

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