Enzyme immunoassay with two-part solution of tetramethylbenzidin

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 25, 435 28, 435 29, 435 39, 435810, 436510, 436531, 436817, 436818, G01N 3354, G01N 3358, G01N 3374, G01N 3376

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045031436

ABSTRACT:
Colorimetric detection of bindable substances such as antibodies and antigens using chromogens of improved sensitivity and stability. The chromogens take the form of activated solutions containing tetramethylbenzidine or water soluble derivatives of tetramethylbenzidine. Such chromogens are of particular use in home testing applications for detection of antigens such as human chorionic gonadotropin, human luteinizing hormone, and gonococcus bacteria, as well as the detection of antibodies for these substances.

REFERENCES:
patent: 3791932 (1974-02-01), Schuurs
patent: 4361648 (1982-11-01), Shuenn-tzong
patent: 4385114 (1983-05-01), Guthlein
Chemical Abstracts, 97: 33481p, (1982).
"Methods in Enzymology", Part A, H. V. Vunakis et al., eds., Chapter 28, by E. Engvall, only pp. 430-432, Academic Press, New York, 1980.

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