Enzyme immunoassay with a macroporous hydrophobic synthetic poly

Details Enzyme immunoassay with a macroporous hydrophobic synthetic poly Enzyme immunoassay with a macroporous hydrophobic synthetic poly

1991-02-08

1992-06-16

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 79, 435 793, 435174, 435180, 435970, 436528, 436531, G01M 33535, G01M 33545, G12N 1108

Patent

active

051224526

ABSTRACT:
An immunoassay device containing an immobilized antibody or antigen is provided by directly absorbing and absorbing an unmodified antibody or antigen on and within a woven or non-woven macroporous hydrophobic synthetic polymer cloth formed of a synthetic polymer selected from the group consisting of plypropylene, polyester, nylon and polyethylene. The cloth has a thickness of more than about 200 .mu.m and contains pores in the form of spaces between fibers exceeding about 20 .mu.m in diameter, and has a Frazier Air Permeability in CFM/ft.sup.2 at 0.5" H.sub.2 O of about 215 for a cloth of thickness of about 40 mils. The cloth has a large surface area for binding to an antibody or antigen and can accommodate a large volume of liquid per surface area and has minimum flow resistance. The cloth containing an immobilized antibody or antigen may be used to carry out an enzyme immunoassay by contacting the cloth with a sample containing an antigen or antibody, incubating the cloth with an enzyme-antibody conjugate and then reacting enzyme bound to the cloth with a chromogenic substrate-indicator to produce a visible color. Other immunoassay embodiments may also be carried out and a control cloth can be used such that a difference in color from that obtained with the control cloth determines the amount of antigen or antibody present in a sample.

REFERENCES:
patent: 4200690 (1980-04-01), Root et al.
patent: 4617326 (1986-10-01), Bjornberg et al.
patent: 4808530 (1989-02-01), Means et al.
Morris et al., Immunoassays in Food Analysis, Elsevier Applied Science Publishers, N.Y., 1985, pp. 53-71.
Yamazaki, et al., Biotechnology letters, vol. 8, No. 2, 1984, pp. 107-110.

Affiliated with

Also associated with

Rating

Enzyme immunoassay with a macroporous hydrophobic synthetic poly does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzyme immunoassay with a macroporous hydrophobic synthetic poly, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme immunoassay with a macroporous hydrophobic synthetic poly will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1753284