Enzyme-immunoassay method for the determination of an analyte

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 793, 435 794, 435 795, 435970, 436518, 436527, 436529, 436530, 436531, 436538, 436541, 436810, 422 56, 422102, G01N 33543

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052963560

ABSTRACT:
The invention concerns a method for the detection of an analyte in a sample liquid by an enzyme-immunoassay in which an enzyme-labelled compound is partitioned between a solid and a liquid phase and the amount of enzyme label in the liquid phase outside the solid phase is determined as a measure of the concentration of the analyte. The measurement is carried out in a non-porous molding having the liquid phase contained therein in contact with the solid phase. The method is particularly suitable for carrying out in a cuvette which can be filled via the porous matrix or on a test strip having a space in contact with the solid phase.

REFERENCES:
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patent: 4446232 (1984-05-01), Liotta
patent: 4654197 (1987-03-01), Lilja et al.
patent: 4729875 (1988-03-01), Chandler
patent: 4743536 (1988-05-01), Evanega et al.
patent: 4786594 (1988-11-01), Khanna et al.
patent: 4857453 (1989-08-01), Ullman et al.
patent: 4978504 (1990-12-01), Nason
Patent Abstracts of Japan vol. 8: NR159 (P. 289) 1596, Jul. 24, 1984.

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