Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-02-19
1993-09-21
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 79, 435 792, 435 793, 435 794, 435 795, 435 8, 435 15, C12Q 100
Patent
active
052468348
ABSTRACT:
The present invention is intended to provide an EIA method utilizing bioluminescence using a firefly luciferase. The EIA method of the present invention is characterized by using an ATP-generating enzyme as a labeling enzyme, subjecting ATP generated to bioluminescence by the use of the firefly luciferase, and measuring the quantity of light emitted. Acetate kinase is a preferred enzyme used in the method.
REFERENCES:
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Fricke, et al. J. Clin. Chem. Clin. Biochem. V. 20 (1982) pp. 91-94.
European Search Report for European Application 92102840.3.
Shutenko, et al., "Adenylate kinase as a marker in immuno-enzyme analysis with a bioluminescence detection system," Chemical Abstracts, 110:244 (1989).
Yabuuchi et al., "Bioluminescence enzyme immunoassay of 17-.alpha.-hydroxyprogesterone using glucose dehydrogenase as label," Bunseki Kagaku, vol. 34 (1985) (English abstract included).
Maeda Masako
Nakajima Moto-o
Tsuji Akio
Kikkoman Corporation
Rosen Sam
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