Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1980-07-02
1982-08-31
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 7, 435177, 435188, 435810, 424 12, 23230B, G01N 3354, C12Q 170, C12N 996
Patent
active
043473113
ABSTRACT:
A highly sensitive enzyme immunoassay procedure is disclosed for determining IgM-, IgA-, IgG- and IgE- antibodies which are specific to antigens by coating a solid support with an antibody which selectively binds the antibody to be determined in a serum specimen and reacting the selectively bound antibody with an enzyme labeled microbe or antigen and then determining the enzyme activity of the reaction product as a measure of the antibody to be detected. As the antigen can be effectively labeled a relatively impure antigen can be labeled and used in high dilutions resulting in a very sensitive assay; purification by column chromatography is unnecessary. A kit for conducting the assay is also disclosed.
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MEDAC Gesellschaft fur Klinishce Spezialpraparate mbH
Wiseman Thomas G.
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