Enzyme immunoassay for detecting HIV antigens in human sera

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 7, 435 7021, 43524027, 4351722, 422 61, 436510, 436518, 436531, 436800, 436805, 436808, 436809, 436811, 436825, 436826, 530387, G01N 33577, G01N 33569

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048867420

ABSTRACT:
A solid-phase immunoassay is provided for determination of HIV antigens in human physiological fluid. The immunoassay is characterized by the coating of a solid substrate with a unique monoclonal antibody which recognizes a common antigenic determinant of a group of HIV core antigens and no HIV envelope antigens of HIV. The test sample preferably also is subjected to a lysing reagent prior to the incubation for uniformly dispersing antigens which may be present in the test sample.

REFERENCES:
patent: 4748110 (1988-05-01), Paul
patent: 4755457 (1988-07-01), Guroff et al.
D. S. Healy, et al., Comparison of enzyme immunoassay and reverse transriptase assay for detection of HIV in culture supernates, J. Virol. Methods 17:237-245 (1987).
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Ferns et al, "Characterization of Monoclonal Antibodies Against the Human Immunodeficiency Virus (HIV) gag Products and Their Use in Monitoring HIV Isolate Variation", J. Gen. Virol, 68 (1987) 1543-1551.
Caruso et al, "Liquid competition radioimmunoassay for the detection and quantitation of the HIV p24", J. Virol. Meth., 17 (1987) 199-210.
Hattori et al, "Characterization of Three Monoclonal Antibodies (VAK3-5) That Identify p24, Core Protein of Human Immunodeficiency Virus, and Its Precursors", Jpn. J. Cancer Res. (Gann), 78 (1987) 235-241.
Ghrayeb et al, "Human T-Cell Lymphotropic Virus Type III (HTLV-III) Core Antigens: Synthesis in Escherichia coli and Immunoreactivity with Human Sera", DNA, 5 (1986) 93-99.
Higgins et al, "Detection and Differentiation by Sandwich Enzyme-Linked Immunosorbent Assay of Human T-Cell Lymphotropic Virus Type III/Lymphadenopathy-Associated Virus- and Acquired Immunodeficiency Syndrome-Associated Retroviruslike Clinical Isolates", J. Clin. Microbiol., 24 (1986) 424-430.
Evans et al, "Human Monoclonal Antibody Directed Against gag Gene Products of the Human Immunodeficiency Virus", J. Immunol., 140 (1988) 941-943.
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