Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1987-11-06
1989-12-12
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 7, 435 7021, 43524027, 4351722, 422 61, 436510, 436518, 436531, 436800, 436805, 436808, 436809, 436811, 436825, 436826, 530387, G01N 33577, G01N 33569
Patent
active
048867420
ABSTRACT:
A solid-phase immunoassay is provided for determination of HIV antigens in human physiological fluid. The immunoassay is characterized by the coating of a solid substrate with a unique monoclonal antibody which recognizes a common antigenic determinant of a group of HIV core antigens and no HIV envelope antigens of HIV. The test sample preferably also is subjected to a lysing reagent prior to the incubation for uniformly dispersing antigens which may be present in the test sample.
REFERENCES:
patent: 4748110 (1988-05-01), Paul
patent: 4755457 (1988-07-01), Guroff et al.
D. S. Healy, et al., Comparison of enzyme immunoassay and reverse transriptase assay for detection of HIV in culture supernates, J. Virol. Methods 17:237-245 (1987).
Kanki et al, "Serologic Identification and Characterization of a Macaque T-Lymphotropic Retrovirus Closely Related to HTLV-III", Science 228 (1985) 1199-1201.
Ferns et al, "Characterization of Monoclonal Antibodies Against the Human Immunodeficiency Virus (HIV) gag Products and Their Use in Monitoring HIV Isolate Variation", J. Gen. Virol, 68 (1987) 1543-1551.
Caruso et al, "Liquid competition radioimmunoassay for the detection and quantitation of the HIV p24", J. Virol. Meth., 17 (1987) 199-210.
Hattori et al, "Characterization of Three Monoclonal Antibodies (VAK3-5) That Identify p24, Core Protein of Human Immunodeficiency Virus, and Its Precursors", Jpn. J. Cancer Res. (Gann), 78 (1987) 235-241.
Ghrayeb et al, "Human T-Cell Lymphotropic Virus Type III (HTLV-III) Core Antigens: Synthesis in Escherichia coli and Immunoreactivity with Human Sera", DNA, 5 (1986) 93-99.
Higgins et al, "Detection and Differentiation by Sandwich Enzyme-Linked Immunosorbent Assay of Human T-Cell Lymphotropic Virus Type III/Lymphadenopathy-Associated Virus- and Acquired Immunodeficiency Syndrome-Associated Retroviruslike Clinical Isolates", J. Clin. Microbiol., 24 (1986) 424-430.
Evans et al, "Human Monoclonal Antibody Directed Against gag Gene Products of the Human Immunodeficiency Virus", J. Immunol., 140 (1988) 941-943.
Cohen et al, "Enumeration of CR1 complement receptors of erythrocytes using a new method for detecting low density cell surface antigens by flow cytometry", J. Immunol. Meth., 99 (1987) 53-58.
Kanner et al, "Human Retroviral env and gag Polypeptides: Serologic Assays to Measure Infection", J. Immunol, 137 (1986) 674-678.
Forman Meryl A.
Hofheinz David E.
Kortright Kenneth H.
Lee Song Y.
Smariga Paulette E.
Cass Myron C.
Coulter Corporation
Nucker Christine M.
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