Enzyme-immunoassay for carcinoembryonic antigen

Details Enzyme-immunoassay for carcinoembryonic antigen Enzyme-immunoassay for carcinoembryonic antigen

1982-08-16

1984-08-21

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

422 61, 435810, 436548, 436813, 436826, G01N 3354

Patent

active

044670311

ABSTRACT:
A solid phase-sandwich enzyme-immunoassay method for the rapid determination of carcinoembryonic antigen (CEA). The sample to be investigated is incubated with a first CEA antibody, which is bound to a water-insoluble carrier, and a second CEA antibody, to which peroxidase is bound directly or via a biotin/avidin bridge. The immunological reaction can be carried out in one or two steps. By the presence of 0.4-1.0 mol/l of phosphate ions, 0.3-0.4 mol/l of sulfate ions or 0.2-0.4 mol/l of tartrate ions in the immunological reaction or in the second step thereof (insofar as it is carried out in two steps) the incubation time period is shortened considerably. After the immunological reaction, the phases are separated, whereupon the peroxidase activity is measured either in the solid or in the liquid phase as the amount of CEA present in the sample.

REFERENCES:
patent: 4180556 (1979-12-01), Kim
patent: 4272504 (1981-06-01), Kim
patent: 4299815 (1981-11-01), Hansen
patent: 4376110 (1983-03-01), David

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