Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1989-03-20
1990-08-14
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 14, 435 15, 435 18, 435 19, 435 21, 435 28, 436536, 436538, 436541, G01N 3353
Patent
active
049487264
ABSTRACT:
An enzyme immunoassay based on membrane separation of antigen-antibody complexes wherein human or animal body fluid specimens containing an antigen are mixed with an enzyme-conjugated antibody specific for the antigen under test. Following incubation the antigen-antibody-conjugate mixture is passed through a filter membrane having an electrostatic charge providing an affinity for retaining antigen-antibody-conjugate complexes while not retaining free antibody-conjugate. Following washing of the filter membrane to remove free antibody-conjugate remaining thereon an enzyme substrate-chromogen reagent solution is applied to the filter membrane, which reacts with filter-bound antibody-conjugate and develops a visible or fluorogenic color indicative of the presence of antibody-conjugate.
REFERENCES:
patent: 4517288 (1985-05-01), Giegel et al.
Jackson James L.
Rosen Sam
LandOfFree
Enzyme immunoassay based on membrane separation of antigen-antib does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Enzyme immunoassay based on membrane separation of antigen-antib, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme immunoassay based on membrane separation of antigen-antib will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-462528