Enzyme immobilzation on a macropermable agglomerate of microporo

Details Enzyme immobilzation on a macropermable agglomerate of microporo Enzyme immobilzation on a macropermable agglomerate of microporo

1991-07-30

1993-07-20

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an...

435180, 435181, C12N 1114, C12N 1108, C12N 1106

Patent

active

052292843

ABSTRACT:
Enzymes are immboilized on an inorganic carrier which is a round, macropermeable agglomerate having a mulberry-type structure formed from spherical microporous .alpha.-aluminum oxide hydroxide particles. An organic polycondensate having free organic functional groups as enzyme binding sites penetrates the agglomerate. The .alpha.-aluminum oxide hydroxide particles of the agglomerate retain essentially their form prior to agglomeration. The polycondensate is prepared by reacting a polyimine with a dialdehyde or an epoxy compound. Preferably, the polyimine is polyethyleneimine, the dialdehyde is glutardialdehyde and the epoxy compound is an epoxy-functional silicon resin, an aliphatic diepoxide or an aliphatic triepoxide. The agglomerate is prepared by granulating the .alpha.-aluminum oxide hydroxide particles in the presence of the polyimine to form the agglomerate having the polyimine dispersed throughout and then reacting the polyimine with the dialdehyde or the epoxy compound. A preferred enzyme is glucose isomerase.

REFERENCES:
patent: 3850751 (1974-11-01), Messing
patent: 4229536 (1980-10-01), De Filippi
patent: 4940664 (1990-07-01), Muecke

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