Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1988-07-08
1990-05-29
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435 94, 435177, 435181, 435182, 435234, C12N 1108, C12N 992, C12N 1104, C12P 1924
Patent
active
049295560
ABSTRACT:
Polysulfonium salts that can react with nucleophilic groups and covalently cross-link are used to immobilize enzymes or enzyme-containing cellular material. Some of the polysulfonium salts can both flocculate and covalently cross-link. Replacement of the cross-linker, glutaraldehyde, with the polysulfonium salt results in greater retention of enzyme activity during immobilization. Immobilization is carried out by forming a mixture of an enzyme or enzyme-containing cellular material and the polysulfonium salt and subjecting the mixture to conditions such that sulfonium ions react with nucleophilic groups contained by the enzyme or cellular material to form a covalently cross-linked and water insoluble product. The enzyme or cellular material may be flocculated with a flocculating agent prior to cross-linking with the polysulfonium salt. The polysulfonium salt can be a polymer containing sulfonium groups.
REFERENCES:
patent: 3935068 (1976-01-01), Nystrom
patent: 4217215 (1980-08-01), Panzer et al.
patent: 4250267 (1981-02-01), Hartdegan et al.
patent: 4337313 (1982-06-01), Hershberger et al.
patent: 4528384 (1985-07-01), Schmidt et al.
patent: 4659665 (1987-04-01), Freeman et al.
patent: 4704324 (1987-11-01), Davis et al.
Zaborsky, O., Immobilized Enzymes, CRC Press, Cleveland, Ohio, 1973, pp. 70-74.
Cheng Roberta C.
Schmidt Donald L.
Wessling Ritchie A.
Naff David M.
The Dow Chemical Company
LandOfFree
Enzyme immobilization with polysulfonium salts does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Enzyme immobilization with polysulfonium salts, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme immobilization with polysulfonium salts will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-520580