Enzyme immobilization with a disulphide bridge

Details Enzyme immobilization with a disulphide bridge Enzyme immobilization with a disulphide bridge

1978-01-13

1979-11-27

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435176, 435174, C12D 1300, C07G 702

Patent

active

041760069

ABSTRACT:
Immobilized enzymes are provided in which the enzyme is covalently linked by a disulphide group-containing bridge to on inorganic or organic support carrier. Immobilizing by this method enables spent immobilized enzyme to be readily regenerated by reduction under mild conditions of the disulphide bridge to provide the carrier with mercaptan groups, and adding fresh enzyme to the carrier. The immobilization method is applicable to a wide variety of enzymes using the same chemical coupling reagents. The method is especially useful for immobilizing expensive enzymes such as glucose isomerase.

REFERENCES:
patent: 3198781 (1965-08-01), Benesch et al.
patent: 3519538 (1970-07-01), Messing et al.
patent: 3846306 (1974-11-01), Barker et al.
patent: 3884761 (1975-05-01), Comling
patent: 3904478 (1975-09-01), Dean et al.
patent: 4008126 (1977-02-01), Keyes
patent: 4069106 (1978-01-01), Stanley et al.
patent: 4113565 (1978-09-01), Hurst

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