Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an...
Patent
1997-07-07
1999-12-07
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an...
435116, 435181, C12N 1114, C12N 1106, C12P 1306
Patent
active
059981830
ABSTRACT:
A simple, efficient method for immobilization of enzymes on siliceous supports is provided. In a preferred embodiment, a siliceous support having surface hydroxyl groups such as silica gel is contacted with a solution of a polyaldehyde cross-linking agent such as glutaraldehyde to produce a modified support containing bound cross-linking agent. After separation from the solution, the modified support is contacted with a solution containing an enzyme to bind the enzyme to free aldehyde functions of the cross-linking agent. Owing to properties peculiar to siliceous supports, the resulting immobilized enzyme is much more stable than enzymes immobilized on other supports. Consequently, much less enzyme is required. The immobilized enzymes generated via this process are ideally suited for continuous enzymatic production of commodity chemicals and pharmaceuticals such as L-DOPA, and for other enzyme-mediated industrial processes.
REFERENCES:
patent: 4071409 (1978-01-01), Messing et al.
patent: 4072566 (1978-02-01), Lynn
patent: 4141857 (1979-02-01), Levy et al.
patent: 4258133 (1981-03-01), Mirabel et al.
patent: 4384045 (1983-05-01), Ho et al.
patent: 4888285 (1989-12-01), Nishimura
patent: 5039377 (1991-08-01), von Raven et al.
Kinrade, et al., "The Peroxysilicate Question. .sup.29 SI-NMR Evidence for the Role of Silicates in Alkaline Peroxide Brightening of Mechanical Pulp", J. Wood Chem. and Technol., 15(2), 203-222 (1995).
Pialis, et al., "L-DOPA Production from Tyrosinase Immobilized on Nylon 6,6", Biotechnology and Bioengineering, vol. 51, pp. 141-147 (1996).
Murray R. Gray, "Substrate Inactivation of Enzymes In Vitro and In Vivo", Biotech. Adv. vol. 7, pp. 527-575, (1989).
J. Gierer, et al., "Formation of Hydroxyl Radicals from Hydrogen Peroxide and Their Effect on Bleaching of Mechanical Pulps", J. Wood Chem. and Technol. 13(4), 561-581 (1993).
Le Fevre Gerard N.
Saville Bradley A.
Mitchell Randall S.
Naff David M.
LandOfFree
Enzyme immobilization on a siliceous support with a polyaldehyde does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Enzyme immobilization on a siliceous support with a polyaldehyde, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme immobilization on a siliceous support with a polyaldehyde will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-822648