Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1981-02-12
1982-10-26
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
2103213, 210927, 435269, 435288, C12N 1112, C12M 140, B01D 1300
Patent
active
043562671
ABSTRACT:
Enzymes are immobilized in a bundle of cellulosic hollow fibres for use as a fibre-bundle dialyzer for purifying blood. Immobilization is carried out by circulating through the lumen of the fibres to activate the fibres an aqueous solution of sodium periodate at a concentration of 0.7-21 mg/ml, a circulation rate of 300-500 mm.sup.3 /minute/mm.sup.2, a temperature of 15.degree.-30.degree. C. and for a period not exceeding 70 minutes, removing excess sodium periodate by washing the fibres, and directly or indirectly anchoring an enzyme to the activated fibres. Washing is preferably carried out by circulating through the lumen of the fibres dionized water followed by a dilute aqueous solution of glycerol. The fibres containing an immobilized enzyme may be sterilized with a sterilizing agent.
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Sundaram et al., Preparation and Properties of Urease Chemically Attached to Nylon, FEBS Letters, vol. 10, No. 5, 1970, (pp. 325-327).
Boniolo Antonio
Callegaro Lanfranco
Denti Ennio
Rosa Umberto
Rossi Valdano
Naff David M.
Sorin Biomedica S.p.A.
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