Enzyme immobilization in a macroporous non-ionic resin

Details Enzyme immobilization in a macroporous non-ionic resin Enzyme immobilization in a macroporous non-ionic resin

1986-02-24

1988-07-12

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435180, 435181, 435182, C12P 1924, C12N 1108, C12N 1106, C12N 1104

Patent

active

047570083

ABSTRACT:
Enzyme-containing microbial cells are contacted with a macro-porous non-ionic resin under reduced pressure below atmospheric to cause the cells to become incorporated into pores of the resin. The resin is then contacted with a multi-functional, amine reactive material to immobilize the cells within the pores. Prior to contacting with the resin, cells may be disrupted to produce a system containing cell fragments, intact cells and solubilized enzyme. A preferred enzyme is glucose isomerase.

REFERENCES:
patent: 3705084 (1972-12-01), Reynolds
patent: 3788945 (1974-01-01), Thompson et al.
patent: 3960663 (1976-06-01), Tamura et al.
patent: 4001085 (1977-01-01), Keyes
patent: 4078970 (1978-03-01), Fujita et al.
patent: 4323650 (1982-04-01), Rosevear
patent: 4504582 (1985-03-01), Swann

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