Enzyme immobilization for electroosmotic flow

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

Reexamination Certificate

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C204S600000, C435S175000, C435S176000

Reexamination Certificate

active

10351976

ABSTRACT:
Disclosed herein is a method and apparatus of immobilizing a biocatalyst on a microfluidic biochip for conducting reactions in the presence of electroosmotic flow. The biochip includes a polymer on its microfluidic flow surfaces, wherein the polymer includes a first substituent selected from ionic groups of the same polarity or precursors thereof, a second substituent that is a hydrophobic group, and a third substituent comprising an immobilized biocatalyst-or precursor thereof. The biochip can be used to conduct multiple sequential biocatalyzed reactions in the presence of electroosmotic flow.

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