Enzyme immobilization and bioaffinity separations with perfluoro

Details Enzyme immobilization and bioaffinity separations with perfluoro Enzyme immobilization and bioaffinity separations with perfluoro

1987-03-02

1989-12-05

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435180, 435815, 436532, 436824, 530413, 530816, C12N 1106, G01N 33547, C07K 1706, C07K 320

Patent

active

048852503

ABSTRACT:
A bioaffinity separation method is provided along with a solid affinity support utilized in that method. Additionally, immobilized enzyme systems are provided for use as enzyme electrode systems. The support is based on an inert perfluorocarbon polymer carrier with ligands or binders attached to its surface. The ligand, binder or enzyme is preferably modified by attaching a perfluorocarbon anchor group, and the modified ligand, binder or enzyme is attached to the carrier through the anchor group. Methods for preparing such supports and their use in capturing target molecules from samples and in analytical applications are also provided.

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Weetall, Methods in Enzymology, vol. XLIV: Immobilized Enzymes, Chapter 10, 134, Ed. K., Mosbach, Academic Press, New York 1976.

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