Enzyme immobilization and bioaffinity separations with perfluoro

Details Enzyme immobilization and bioaffinity separations with perfluoro Enzyme immobilization and bioaffinity separations with perfluoro

1987-12-17

1990-09-04

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435 7, 435180, 435803, 435815, 435817, 436531, 436532, 436824, 530815, 530816, C12N 1106, C12N 1108, G01N 33549, C07K 1708

Patent

active

049544446

ABSTRACT:
A bioaffinity separation method is provided along with a solid affinity support utilized in that method. Additionally, immobilized enzyme systems are provided for use as enzyme electrode systems. The support is based on an inert perfluorocarbon polymer carrier with ligands or binders attached to its surface through a highly fluorinated isocyanate anchor group. Methods for preparing such supports and their use in capturing target molecules from samples and in analytical applications are also provided.

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Danielson et al., Biotechnology and Bioengineering, 23, 1919-1917 (1981).
Weetall, Methods in Enzymology, vol. XLIV: Immobilized Enzymes, Chapter 10, 134, Ed. K, Mosbach, Academic Press, New York (1976).

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