Enzyme immobilization

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an...

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435174, 435177, C12N 1114

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active

042660297

ABSTRACT:
An immobilized enzyme granular product suitable for use in expanded and fluidized bed operations is formed by gelatine coating a dense particulate material, e.g., a mineral oxide, and hardening the coating with glutaraldehyde, then treating the coated particles with a pasty mixture of an enzyme substance and polyethylene imine, and thereafter treating the mixture with glutaraldehyde, followed by granulating and drying. Sand and titanium dioxide are preferred particulate materials. Lactase, urease, and inulinase are preferred enzymes, preferably in the form of whole or homogenized microbial cells.

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patent: 3928143 (1975-12-01), Coughlin
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patent: 4138290 (1979-02-01), McMullen et al.
patent: 4141857 (1979-02-01), Levy et al.
Grittith, et al., A New Method for Coating Fermentation Tomer Packing So as to Facilitate Microorganism Attachment, Developments in Industrial Microbiology, vol. 17, 1976 (pp. 241-246).
Leroy et al., Insoluble Particles with Enzymic Activity, Chemical Abstracts, vol. 88: 59763w, 1978 (p. 164).
Hartmeier et al., Carrier-Fixed Enzymes Chemical Abstracts, vol. 88: 117655e, 1978 (p. 207).
Miyairi et al., Immobilization of Enzyme by Azide Compound, Chemical Abstracts, vol. 89: 19233n, 1978 (p. 257).

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