Enzyme gene participating in the synthesis of polyester and...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S254200, C435S483000, C435S254210, C435S254220, C435S254230, C536S023200

Reexamination Certificate

active

07429469

ABSTRACT:
This invention relates to a method of producing polyesters resulting from homopolymerization or copolymerization of a 3-hydroxyalkanoic acid(s) and having biodegradability and good physical properties using yeasts as hosts. By constructing at least one enzyme gene involved in polyester synthesis by adding a DNA coding a peroxisome-targeting signal, introducing an enzyme gene expression cassette containing that gene into yeast, and cultivating the thus-obtained transformant, it becomes possible to cause accumulation of a polyester resulting from homopolymerization or copolymerization of a 3-hydroxyalkanoic acid(s) in yeast cells and recover the polyester from the culture.

REFERENCES:
patent: 0 824 148 (1998-02-01), None
patent: 57-150393 (1983-07-01), None
patent: 59-220192 (1984-07-01), None
patent: 5-93049 (1993-03-01), None
patent: 7-265065 (1995-10-01), None
patent: 10-108682 (1998-02-01), None
patent: 2002-262886 (2003-12-01), None
patent: WO 9935278 (1999-07-01), None
patent: WO 00/43525 (2000-07-01), None
patent: WO 01/88144 (2001-11-01), None
Fukui et al. J. Bactrol. 1997, pp. 4821-4830.
T. Fukui et al., “Cloning and Analysis of the Poly(3-Hydroxybutyrate-co-3-Hydroxyhexanoate)Biosynthesis Genes of Aeromonas caviae”, Journal of Bacteriology, 179(15):4821-4830 (1997).
E. H. Hettema et al., “Import of proteins into peroxisomes”, Biochimica et Biophysica Acta, 1451:17-34 (1999).
K. Kondo, “Kobo Candida utilis no Ishu Idenshi Hatsugenkei”, Kagaku to Seibutsu, 38(9):614-620 (2000) with summary in English.
T. A. Leaf et al., “Saccharomyces cerevisiae expressing bacterial polyhydroxybutyrate synthase produces poly-3-hydroxybutyrate”, Microbiology, 142:1169-1180 (1996).
V. Mittendorft et al., “Synthesis of medium-chain-length polyhydroxyalkanoates in Arabidopsis thaliana using intermediates of peroxisomal fatty aicd β-oxidation”, Proc. Natl. Acad. Sci, USA, 95:13397-13402 (1998).
Y. Poirier et al., “Synthesis of Polyhydroxyalkanoate in the Peroxisome of Saccharomyces cerevisiae by Using Intermediates of Fatty Acid β-Oxidation”, Applied and Environmental Microbiology, 67(11):5254-5260 (2001).
Q. Ren, et al., “FabG, and NADPH-Dependent 3-Ketoacyl Reductase of Pseudomonas aeruginosa, Provides Precursors for Medium-Chain-Length Poly-3-Hydroxyalkanoate Biosynthesis in Escherichia coli”, Journal of Bacteriology, 182(10):2978-2981 (2000).
Sequence Listing from WO 01/88144 A3 (XP-002377462).
Sequence Listing from WO 01/88144 A4 (XP-002377463).
Sequence Listing from Fukui, T., et al., “Cloning and Analysis of the Poly(3-Hydroxyalkanoate-Co-3-Hydroxyalkanoate) Biosynthesis Genes of Aeromonas Caviae”,Journal of Bacteriology, vol. 179, No. 15, Aug. 1997, pp. 4821-4830. (XP-002377464).
Fukui, T., et al., “Co-Expression of Polyhydroxyalkanoate Synthase and (R)-Enoyl-Coa Hydratase Genes ofAeromonas CaviaeEstablishes Copolyester Biosynthesis Pathway inEscherichia coli”, FEMS Microbiology Letters, vol. 170, 1999, pp. 69-75.
Subramani, Suresh, “Protein Import into Peroxisomes and Biogenesis of the Organelle”,Annual Review of Cell Biology, vol. 1993, pp. 445-478.
Allenbach, Laure, et al., “Analysis of the Alternative Pathways for the β-Oxidation of Unsaturated Fatty Acids Using Transgenic Plants Synthesizing Polyhydroxyalkanoates in Peroxisomes”,Plant Physiology, vol. 124, No. 3, Nov. 2000, pp. 1159-1168.
Miura, Y., et al., “Production of the Carotenoids Lycopene, β-Carotene, and Astaxanthin in the Food YeastCandida utilis”, Applied and Environmental Microbiology, Apr. 1998, vol. 64, No. 4, pp. 1226-1229.

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