Enzyme extract from germinated sorghum for hydrolysis of protein

Details Enzyme extract from germinated sorghum for hydrolysis of protein Enzyme extract from germinated sorghum for hydrolysis of protein

1988-12-16

1991-06-04

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435219, 426 47, 426 49, 426 52, 530343, C12P 2106, C12N 950, A23J 300, A61K 3702

Patent

active

050213385

ABSTRACT:
An enzyme system for hydrolysis of protein material from plant and animal sources is obtained by extracting germinated sorghum seeds. The enzyme system contains a proteolytic component active on intact proteins and large peptides at an optimum pH of 3.2 to 4.2 and a peptidase component active on small peptides at an optimum pH of 4.5 to 6.5. A protein hydrolyzate having an average molecular weight within a selected range can be obtained by varying the pH so as to depress activity of the proteolytic component or the peptidase component. The protein hydrolysates which are obtained are characterized by the absence of bitter taste. Furthermore, by suitably adjusting the enzyme concentration in the reaction medium, the temperature and the hydrolysis time, protein hydrolyzates with an average molecular weight lower than 700 and a low percentage of free amino acids can be obtained.

REFERENCES:
patent: 3857966 (1974-12-01), Feldman et al.
patent: 4452888 (1984-06-01), Yamazaki et al.
Garg et al., Eur. J. Biochem., vol. 17, No. 1, 1970, pp. 4-12, 13-18.

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