Enzyme electrode for measuring malto-oligosaccharide

Details Enzyme electrode for measuring malto-oligosaccharide Enzyme electrode for measuring malto-oligosaccharide

1989-03-09

1992-01-14

Lacey, David L.

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

435175, 435817, 435913, 435939, 204403, C12M 140

Patent

active

050810375

ABSTRACT:
An enzyme electrode for measuring malto-oligosaccharides having a co-immobilized enzyme membrane containing glucoamylase and a glucose oxidizing enzyme such as glucose oxidase or pyranose oxidase.
The glucoamylase and glucose oxidizing enzyme are immobilized at a ratio of ##EQU1## where Va is the maximum reaction rate of glucoamylase as expressed in the glucose formation rate from maltose, and Vo is the maximum reaction rate of glucose oxidizing enzyme as expressed in the hydrogen peroxide formation rate from glucose.
In such enzyme electrode, malto-oligosaccharides are detected at the response value proportional to their degree of polymerization. Therefore, the malto-oligosaccharides can be measured at the glucose converted concentration.

REFERENCES:
patent: 4172765 (1979-10-01), Keyes
patent: 4340448 (1982-07-01), Schiller et al.
patent: 4810642 (1989-03-01), Aoyama et al.
Inman et al., Biochem. J., 137, 25-32 (1974).
Patent Abstracts of Japan, vol. 10, No. 183 (P-472) [2239], 6/26/86.
Patent Abstracts of Japan, vol. 10, No. 344 (P-518) [2400], 11/20/86.
Pfeiffer et al., Biochimie, No. 62, 1980, pp. 587-593.
Chemical Abstracts, 102: 181456W, 1985.
Walker, J. M. and Cox, M., The Language of Biotechnology A Dictionary of Terms, Maple Press Co., 1988, p. 111.

Affiliated with

Also associated with

Rating

Enzyme electrode for measuring malto-oligosaccharide does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzyme electrode for measuring malto-oligosaccharide, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme electrode for measuring malto-oligosaccharide will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-541034