Enzyme electrode and process for preparation thereof

Details Enzyme electrode and process for preparation thereof Enzyme electrode and process for preparation thereof

2007-09-11

2007-09-11

Noguerola, Alex (Department: 1753)

Coating processes

Medical or dental purpose product; parts; subcombinations;...

Analysis, diagnosis, measuring, or testing product

C204S403140

Reexamination Certificate

active

10345163

ABSTRACT:
The present invention relates to an enzyme electrode useful for estimation of cholesterol in aqueous medium, said electrode comprising:i. an electrically conductive base plate,ii. a film of sol gel derived material deposited thereon,said sol gel derived material of step b) being microencapsulated cholesterol oxidase with an electron mediator. The present invention also relates to a process for the preparation of an enzyme electrode by coating an immobilized cholesterol oxidase (ChOx) and mediator on a silicate sol gel by microencapsulation.

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CAPLUS abstract of Li et al. (“Polyaniline/Prussian blue composite film electrochemical biosensors for cholesterol detection,” Chinese Journal of Chemistry (2002), 20(10), 1038-1043).
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Brahim et al. (“Amperometric determination of cholesterol in serum using a biosensor of cholesterol oxidase contained within a polypyrrole-hydrogel membrane,” Analytica Chimica Acta 448 (2001) 27-36).
Niu et al. (“Reagentless mediated biosensors based on polyelectrolyte and sol-gel derived silica matrix,” Sensors and Actuators B 82 (2002) 250-258).
CAPLUS abstract of Dong (CN 1118376 A) Mar. 13, 1996.
CAPLUS abstract of Watanabe et al. (WO 2002057767 A1) Jul. 25, 2002.
Kumar et al. “Co-Immobilization of Cholesterol Oxidase and Horseradish Peroxidase in a Sol-gel Film”, Analytica Chimica Acta, vol. 414, No. 1-2, Jun. 1, 2000, pp. 43-50, XP002251005.
Wang, “Sol-gel Materials for Electrochemical Biosensors”, Analytica Chimica Acta, vol. 399, No. 1-2, Nov. 8, 1999, pp. 21-27, XP002251371.
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