Enzyme electrode

Details Enzyme electrode Enzyme electrode

2007-01-30

2007-01-30

Tsang, Cecilia J. (Department: 1654)

Chemistry: electrical and wave energy

Apparatus

Electrolytic

C324S448000, C205S777500

Reexamination Certificate

active

10344340

ABSTRACT:
Disclosed is an enzyme electrode comprising an electrode system comprising an insulating substrate having formed thereon a working electrode, a counter electrode and optionally a reference electrode, and a reaction layer formed on the electrode system, wherein the reaction layer comprises diaphorase (DI), 12α-hydroxysteroid dehydrogenase (12α-HSD) and nicotinamide adenine dinucleotide synthetase (NADS), at least a portion of the reaction layer being superposed on the working electrode, wherein the DI, 12α-HSD and NADS contained in the reaction layer are immobilized on the surface of the working electrode, so that a compound generated in the reaction layer can reach the surface of the working electrode. Also disclosed are a method for and a system for determining the concentration of adenosine triphosphate (ATP) in a sample by using the above-mentioned enzyme electrode.

REFERENCES:
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C.K. Yu and L.S. Dietrich (1972) “Purification and properties of yeast nicotinamide adenine dinucleotide synthetase,” Journal of Biological Chemistry vol. 247, pp. 4794-4802.
“Glucose oxidase/hexokinase electrode for the determination of ATP1” by Dario Compagnone et al.,Analytica Chimica Acta, 1997, vol. 340, pp. 109-113.
Fukyuban Sensa Gijyutu (Sensor Technology, Popularized version), published by Fuji Technosystem Co. Ltd., Japan (1976), p. 332.
“Enzyme Electrodes for ADP/ATP with Enhanced Sensitivity due to Chemical Amplification and Intermediate Accumulation”,Electroanalysis, 1991, vol. 3, pp. 569-663.

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