Enzyme electrochemical sensor electrode and method of making it

Details Enzyme electrochemical sensor electrode and method of making it Enzyme electrochemical sensor electrode and method of making it

1989-12-11

1992-01-21

Kaplan, G. L.

Chemistry: electrical and wave energy

Apparatus

Electrolytic

20415312, 435182, 435288, 435817, G01N 2730

Patent

active

050825500

ABSTRACT:
An electrochemical sensor electrode is formed from an electronic conductor coated with a casting solution containing a perfluorosulfonic acid ionomer and a selected enzyme. The selected enzyme catalyzes a reaction between a predetermined substance in a solution and oxygen to form an electrochemically active compound that is detected at the electronic conductor. The resulting perfluorosulfonic acid polymer provides a stable matrix for the enzyme for long lived enzyme activity, wherein only thin coatings are required on the metal conductor. The polymer also advantageously repels interfering substances from contacting the enzyme and contains quantities of oxygen to maintain a sensing capability during conditions of oxygen depletion in the sample. In one particular embodiment, glucose oxidase is mixed with the perfluorosulfonic acid ionomer to form an electrode for glucose detection.

REFERENCES:
patent: 4415666 (1983-11-01), D'Orazio et al.
patent: 4418148 (1983-11-01), Oberhardt
patent: 4517291 (1985-05-01), Seago
patent: 4604182 (1986-08-01), Seago
D. Jed Harrison et al., "Characterization of Perfluorosulfonic Acid Polymer oated Enzyme electrodes and a Miniaturized Integrated Potentiostat for Glucose Analysis in Whole Blood," 60 Anal. Chem., No. 19, pp. 2002-2007 (Oct. 1, 1988).

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