Enzyme cycling based assays for alpha-methylacyl-CoA racemase

Details Enzyme cycling based assays for alpha-methylacyl-CoA racemase Enzyme cycling based assays for alpha-methylacyl-CoA racemase

2008-05-20

2008-05-20

Gitomer, Ralph (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

C435S025000, C435S007910

Reexamination Certificate

active

07374897

ABSTRACT:
The present invention provides a method for assaying alpha-methylacyl-CoA racemase activity. In the assay, a sample containing an alpha-methylacyl-CoA racemase or suspected of containing an alpha-methylacyl-CoA racemase is contacted with (2R)-pristanoyl-CoA. If alpha-methylacyl-CoA racemase is present in the sample, (2R)-pristanoyl-CoA is converted into (2S)-pristanoyl-CoA. The method then utilizes a cycling reaction system between (2S)-pristanoyl-CoA and trans-2,3-dehydropristanoyl-CoA to generate a detectable signal that corresponds to the alpha-methylacyl-CoA racemase activity. Kits for assaying alpha-methylacyl-CoA racemase based on the same principle are also provided.

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