Enzyme-coupled ultrafiltration membranes

Textiles: fiber preparation

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195 63, 195DIG11, 195116, 426 34, C07G 702

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active

040338223

ABSTRACT:
Pressure-driven conditions are used to activate and couple enzymes to ultrafiltration membranes such as a homoporous cellulosic membrane having pores of about 15-200 angstroms in diameter. The resultant enzyme-coupled ultrafiltration membranes can be used under pressure-driven conditions for enzymatic conversion of a substrate. The rate of conversion at low substrate concentrations is larger than the rate achieved with the native enzyme.

REFERENCES:
patent: 3645852 (1972-02-01), Axen et al.
patent: 3705084 (1972-12-01), Reynolds
patent: 3808305 (1974-04-01), Gregor
Thang, et al., Observations on the Activity of Enzymes After Filtration and through) A Nitrocellulose Membrane, Biochem. Biophys. Res. Comm., vol. 31, No. 1, 1968, pp. 1-8.
Inman, et al., The Immobilization of Enyzmes on Xylon Structures and their Use in Automated Analysis, Biochem. J. vol. 129, 1972, pp. 255-262.

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